BD Accuri C6 Flow Cytometer

Flow Cytometers
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The BD Accuri C6 Flow Cytometer brings users accessible, simple to maintain, affordable personal flow cytometry where they need it. The BD Accuri C6 is a powerful compact instrument, and at 30lbs, it can be easily placed just about anywhere it’s needed, whether at a workbench or in a laminar hood.  This flow cytometer is rugged and desgined to withstand harsh conditions, even when in motion, if the machine is anchored down, it could be used on a ship.

The BD Accuri C6 Flow Cytometer comes with a 2 laser system, red and blue, with two light scatter detectors, and four fluorescence detectors with optical filters optimized for the detection of FITC, PE, PerCP, and APC. The optical design of this flow cytometry system is fixed alignment that’s been preoptimized, making it one of the easiest to use.  In fact the lasers and alignments are set and locked down at manufacturing, voltage adjustments to this flow cytometer are not required.

The BD Accuri C6 Flow Cytometer features intuitive software interfaces, templates and reagent kits making it simple to use no matter the user’s skill set. The BD Accuri C6 stores robust data collected that can be accessed for reanalysis at any time, over 7.s decades worth( 16 million channels of digital data). This Flow Cytometer comes with a quick start guide to get user’s up and running right away, as well as intuitive software to help guide them through their work flow. Once data has been collected, it may be visualized at any scale, letting users set gates and regions they need with precision.  The software sends users easy to read and understand messages about the status of their runs.

This BD Accuri C6 Flow Cytometer is equipped with the optional BD C Sampler, that gives users an added element of automation, and walk away convenience. This system is designed to support 48 and 96 well plates as well was deep well plates and a 24 tube rack. The sampler when paired with the flow cytometer only takes about three feet of space, keeping the foot print still small. Should a sample need immediate processing, the current run can be paused, and after the operation is complete, can be resumed where it left off. Contact us today to learn more about our BD Accuri C6 Cytometry System.

BD Accuri C6 Flow Cytometer Specifications:


Laser Excitation

488 nm, 640 nm

Laser Profile 

10 x 75 μm

Light Scatter Detection

Forward (0°, ±13°)

Side (90°, ±13°)

Emission Detection

 4 colors, user-changeable optical filters

Standard set installed

FL1 533/30 nm (eg, FITC/GFP)

FL2 585/40 nm (eg, PE/PI)

FL3 >670 nm (eg, PerCP, PerCP-Cy5.5, PE-Cy™7)

FL4 675/25 nm (eg, APC)

Optical Alignment

Fixed alignment


Fluorescence Sensitivity, MESF

FITC <75; PE <50

Scatter Resolution

Resolves human peripheral blood lymphocytes, monocytes, and granulocytes

Fluorescence Linearity

2 ±0.05% for chicken erythrocyte nuclei (CEN)

Fluorescence Precision

<3% CV for CEN

Data Acquisition Rate

Up to 10,000 events/second

Signal Processing   

24-bit data path


Flow Cell 

200-μm ID quartz capillary

Minimum Detectable Particle Size

0.5 μm

Minimum Sample Volume 

50 μL

Pre-Set Flow Rates and Core Sizes

Slow: 14 μL/min, 10-μm core

Medium: 35 μL/min, 16-μm core

Fast: 66 μL/min, 22-μm core

Custom Sample Flow Rates

10–100 μL/min

Custom Core Diameter

 5–40 μm

Recommended Sheath Fluid 

0.2-μm filtered DI water with BD Sheath Additive
Maximum Events Per Sample 1 million events

Fluid Bottle Capacity                         

2-L sheath fluid

2-L waste

250-mL cleaner

250-mL decontamination fluid

Data Management


8 USB ports (2 USB 3.0 side, 4 USB 3.0 and 2 USB 2.0 rear)


Intel Dual Band Wireless-N 7260 2x2 AC

Bluetooth 4.0

Computer Interface

USB 2.0 or USB 3.0

Installation Requirements

Power Requirements

100–240 VAC, 50/60 Hz

Typical Power Consumption

154 VA

Heat Output

240 BTU/hour maximum output

Operating Ranges

15–30°C; <80% relative humidity

Instrument Size (H x W x D)

 11 x 14.75 x 16.5 in. (27.9 x 37.5 x 41.9 cm)

Footprint with Fluid Bottles (H x W x D)  

11 x 21.5 x 16.5 in. (27.9 x 54.6 x 41.9 cm)


 30 lb (13.6 kg)

  • BD Accuri C6 Flow Cytometer 2013 System
  • C Sampler 
  • Windows 7 Computer Workstation
  • C Flow Plus Software
  • C Flow Sampler Software
  • Waste Container
  • Sheath Container
  • Cleaning Fluid Container
  • Decontamination Fluid Container
  • Pack on Inline Sheath Filters
  • Communication Cable
  • Power Cord
  • 8 & 6 Peak Validation Report
  • Operator's Guides
  • Original Packaging
  • Warranty

This BD Accuri C6 Flow Cytometer has been inspected top to bottom and calibrated to factory specifications, and it performed beautifully. We ran an 8 peak bead validation kit through it and system passed with a clean bill of health. All of our used lab equipment comes backed with our standard warranty. Should you have any questions about this instrument, please don't hesitate to contact us.

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