BioTek Synergy HT Multi Mode Microplate Reader

Microplate Readers
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The BioTek Synergy HT is a multi-detection microplate reader designed to read in fluorescence, absorbance and luminescence.  The Synergy HT SIAFR is equipped with dual optics for unmatched performance in all forms of detection.  This Biotek microplate readers uses a tungsten halogen lamp combined with filters and a photo multiplier tube (PMT) when running in fluorescence.  This method provides large sums of constant light giving the highest level of sensitivity and repeatability.  The filters inside of the BioTek Synergy HT are designed to prevent against light leakage form excitation to emission wavelength.  Scientists have the ability to customize their measurements with the different filters and band passes on the BioTek Synergy.  When running in Luminescence, the low noise PMT operates in a digital photon mode for optimal detection.  If your using the BioTek Synergy HT in absorbance mode the microplate reader switches to different Xenon Flash Lamp which allow the detection of UV and visible light. 

Combined with a monochromator for wavelength selection, the Xenon Flash Lamp used in the BioTek Synergy delivers high performance results in a matter of seconds.  The monochromator affords you the opportunity to select from 200 to 999 nanometers in 1nm increments.  In addition, to being a leading multi-mode microplate reader, the Bio-Tek Synergy HT also comes equipped with a temperature control system allowing end users the best temperature uniformity needed for kinetic assays.  The sleek and compact design of this microplate reader, combined with its multi-detection abilities makes the BioTek Synergy a must have for every lab.  In addition, the Synergy HT is also compatible with a wide range of automated systems included the Bio-Tek BioStack system.  The SIAFRT model can reads Time Resolved Fluorescence as well as bottom reading and runs on Gen5 software.

BioTek Synergy HT System Specifications:

Detection ModeAbsorbance (UV/Vis), Luminescence, Fluorescence and Time Resolved Fluorescence
Read MethodKinetic, End Point, Spectral Scanning, Well Area Scanning
Temperature Control4°C above ambient to 50°C ± 0.5°C at 37°C
Shaking Yes, Built-in
Microplate Formats6 to 384 well plates, PCR plates, accepts Take3 multi-volume plate with 2µL microspots

 Absorbance Specifications:

Wavelength SelectionMonochromator
Wavelength Range200 - 999nm, 1nm increments
Bandpass2.4 nm
Dynamic Range0 - 4.0 OD
Resolution0.001 OD
Monochromator Wavelength Accuracy±0.2nm
Monochromator Wavelength Repeatability±0.2nm

Fluorescence Specifications:

SensitivityFluorescein 5 pM typical
Wavelength SelectionDeep blocking filters
Wavelength Range300 - 700 nm (200 - 850 nm option)
Dynamic Range5 decades
Detection SystemPMT
Reading Speed96 well at 31 seconds, 384 wells at 80 seconds

Luminescence Specifications:

Sensitivity 30 amol ATP typical (flash)
Wavelength Range300 - 700 nm
Dynamic Range> decades
Detection systemLow noise PMT

Biotek Synergy HT System Breakdown:

Power 100 - 240 VAC 50/60Hz
Dimensions16" x 15" x 10" (L x W x H)
Weight40 lbs

  • Bio Tek Synergy HT SIAFR
  • Winows 7 Notebook
  • 115V Power Cord
  • Operator's Guide
  • IQ Absorbance Validation Report
  • IQ Fluorescence Validation Report
  • Monochromator 
  • Excitation Filter
  • Communication Cables
  • 30-Day Warranty

This listing is for a fully refurbished BioTek Synergy HT plate reader model SIAFRT.  Instrument has been calibrated to the Gen5 computer workstation.  Microplate Reader has been tested to perform at factory specification and is currently ready to be shipped.  System is completely refurbished and backed with our standard warranty.  If you would like to schedule a site inspection don't hesitate to contact us.  If you have any questions about any of our used lab equipment listing don't hesitate to give us a call at 617-999-5746.  If you or any of your colleagues has surplus equipment sitting around getting old on your dime please let us know.  We accept trade-in an offer a variety of asset management services including lab buyouts.  Sell Your Lab Equipment Here.

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