BioRad CFX96 Real Time PCR

PCR Thermal Cyclers
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The Biorad CFX96 Real-Time delivers accurate quantification for up to five targets within large volume reactions.  The qPCR can also perform target discrimination as well.  The Bio-Rad CFX96 delivers the latest Real-Time PCR technology while providing a platform which can run as a stand alone system, or software driven if so desired.  In order to perform reproducible results, each sample well of the Real-Time PCR must maintain uniform temperature throughout each incubation step.   The BioRad CFX96 does so by utilizing it's six independent thermal electric modules which are controlled either through the CFX Manager software, or by the built-in microprocessor controls provided with this qPCR. These controls not only manage heating, but also the cooling element of this system, even during ramping of the Thermal Cycler. The BioRad CFX96 joins the company of the Bio-Rad CFX96 Touch and Deepwell Touch as one of the highest sought after Real-Time PCR systems on today's market.  It's optical reaction module converts the BioRad C1000 LCD  thermal cycler chassis into the powerful and precise system which dramatically shortens thermal cycle run times into minutes in stead of hours.  The CFX Manager provided with the BioRad CFX96 is extremely easy to use and comes equipped with built-in protocols required for Real-Time PCR reactions.  

Reagents can be purchased at Bio-Rad along with Applied Biosystems as some of their PCR reagents work with this system. The CFX Manger software from BioRad allows you to set the optimal temperature of your experiments by monitoring and tracking results in real-time using the built-in gradient thermal cycle feature.  With the BioRad CFX96 you can program up to 24°C throughout the reaction block during any step in your assay.  This provides unsurpassed temperature uniformity along with reproducibility in every gradient zone. In addition, the Bio-Rad CFX96 Real-Time PCR allows for quick setup with factory calibrated dyes.  Users can easily monitor real-time data on the LCD Screen, or within the CFX Manager software.  Having this feature allows the user to make quick adjustments to your protocols which in the end will result in higher productivity.  Moreover, the data collected from the qPCR system can easily be modified and quickly exported to a number of different file extensions.  All in all this system will allow you toquickly set up runs and monitor amplification traces in real-time on the integrated C1000 base with the Bio-Rad CFX96.   The system is extremely easyt to use, power and will make for high quality results.  Contact us today to learn more about the BioRad CFX96 Real-Time PCR.

BioRad CFX96 Real-Time PCR Specifications:

Max. ramp rate5°C / per second
Average ramp rate3.3°C / per second
Heating & Cooling MethodPeltier
LidHeats up to 105°C
Temperature Range0-100°C
Temperature Accuracy±0.2 of programmed target at 90°C
Temperature Uniformity ±0.4 well-to-well within 10 sec of arrival at 90°C
CFX96 qPCR Gradient Operational Range30 - 100°C
Gradient Programmable Span1 - 24°C
Optical Detection Excitation6 Filtered LEDs
Optical Detection6 Filtered Photodiodes
Range of Excitation / Emission Wavelengths450 - 730 nm

Detects 1 copy of target sequence

in human genomic DNA

Dynamic Range10 orders of magnitude
Scan Time All Channels12 seconds
FAM/SYBR Green only on this Real-Time PCR3 seconds
Sample Capacity 96 wells
Sample Size1-50µl (10 - 25 recommended)
Communication InterfaceUSB 2.0
Dimnesions  33 (w) x 46 (d) x 36(h) cm
Dimensions13" x 18" x 14" inches
Weight21kg / 47lb

  • BioRad CFX-96 Real-Time PCR System
  • BioRad C1000 Base
  • Windows 7 Laptop Running CFX Manager Software
  • Power Cord
  • Operator's Guide
  • USB Cable
  • Warranty

This listing is for a used Bio-Rad CFX96 Real-Time PCR.  System has been tested by our technician to ensure the qPCR is performing at BioRad's listed specifications.  Instrument is being offered for a fraction of the cost new and comes complete with a Windows 7 notebook running the latest version of Bio-Rad CFX Manager software.  This machine has the option to run independently without the software if so desired. Our turnaround time for this instrument is 3-5 business days.  The BioRad CFX96 qPCR will ship out via freight so please let us know if you need a lift gate at time of delivery.  If you have any question about our real-time PCR machines please give us a call at 617-999-5746.  Should you have any BioRad CFX Real Time PCR Systems for sale please let us know. We're always looking to improve on our used biotech equipment inventory and we routinely purchase these models along with ABI StepOne Plus, ABI 7500, Qiagen Rotogen, and Bio-Rad CFX384 system to meet the needs of our customers.  If you have any of these machines sitting around collecting dust please give us a call.

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