ABI 7300 Real Time PCR

  • The ABI 7300 Real Time PCR is available from The Lab World Group, sellers of quality used lab equipment.
  • The ABI 7300 Real Time PCR is an affordable solution for labs with a small budget
  • The ABI 7300 Real Time PCR has a four-color detection system combines thermal cycling with fluorescence detection
  • The ABI 7300 Real Time PCR measures cycle by cycle accrual
  • The ABI 7300 Real Time PCR  gives end users the flexibility to run a variety of application including SNP genotyping, gene expression, plus/minus assays, pathogen quantitation and more
  • The ABI 7300 Real Time PCR is small yet robust and comes equipped with a CCD camera for precision optics
  • The ABI 7300 Real Time PCR advanced multi-component algorithms for accurate and reproducible results
  • The ABI 7300 Real Time PCR can accommodate standard 96-well pates and 0.2 ml tubes
  • The ABI 7300 Real Time PCR and works with several different dye choices
  • The ABI 7300 Real Time PCR uses control software with built in simple step-by-step wizard instruction
  • The ABI 7300 Real Time PCR is Peltier based
  • The ABI 7300 Real Time PCR holds a 96 well block
  • The ABI 7300 Real Time PCR has a sample ramp rate of 1.1 degree per second


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The ABI 7300 is a reliable tool which combines thermal cycling with fluorescence detection that measures cycle by cycle accrual.  This small but robust PCR takes up less space than the Applied Biosystems 7900HT and is great quantifying nucleic acid sequences without compromising data.  In addition, the ABI 7300 works with a number of different dye choices. The Applied Biosystems 7300 Real Time PCR runs on its own software platform and quantitative results are available immediately, once PCR run is complete.  The great thing about the ABI 7300 real-time PCR is there’s no need to purify PCR, run any gels, or implement post PCR alterations.  Complete Real-Time PCR runs in less than two hours with the ABI 7300 real time using tubes, 96 well plates, 8-strip tubes.  The acceptable volume range is 20-100µl which saves time when compare to manual PCR application such as RNase protection, Northern blotting. 

The Applied Biosystems 7300 delivers four color detect and has the ability to run multiple applications including SNP genotyping, pathogen quantification, isothermal, gene expression analysis, along with plus/minus assays which use internal positive controls.  The ABI qPCR software control software comes equipped with simple step-by-step wizard instruction to help you set up your assways.  In addition, the Applied Biosystems 7300 and ABI 7500 SDS software gives you the ability to sort, graph and export data to extenisions such as Microsoft PowerPoint.    Achieve powerful and reliable results with the precision optics and chargecoupled CCD camera from the ABI 7300.  

Some of additional Real-Time PCR applications include Realative Quantitation (RQ) assays, Absolute Quantitation using standard curves and Post PCR detecion of nucleic acids.  When it comes to fluorescence detection the ABI 7300 and Applied Biosystems 7500 deliver.  Each sample wells are lit up with a tungsten halogen lamp.  There are four filters buit into the 7300 which allows for fluorescence emission and optimized for use with fluorescent dyes such as NED, TAMRA, SYBR Green, VIC, JOE and FAM.    View the progress of your amplification growth curves with a simple clicks of the mouse through the SDS software for the 7300 and ABI 7500 systems.  Contact us today to learn more about our ABI Real-Time PCR Machines. 

Applied Biosystems 7300 Specifications:

Thermal cycling system

Peltier-based, 96-well block

Sample Ramp Rate

± 1.1°C/sec

Peak Block Ramp Rate


Temperature Range

4°C – 100°C

Temperature Accuracy

± 0.25°C of setpoint / display temperature measured at 3 minutes after clock start

Temperature Uniformity

±0.50°C, 30 seconds after clock start

Optical system

Single excitation, four emission filters, and CCD camera

Calibrated Dyes at Installation


Passive Reference Dyes 

ROX or any calibrated dye

Data Collection

Data collected in all 4 filters for all wells regardless of plate setup. Plate setup may 

Quantitative PCR run time

< 1 hour 50 minutes

Supported Volumes

20 – 100 μL

Supported Consumables 

Standard optical 96 well plates
0.2ml tubes
Optical adhesive covers
Optical flat caps
8-strip 0.2ml tubes 


115V 50/60Hz


17" x 13" x 19"


75 lbs

  • ABI 7300 Real Time PCR System
  • Windows 7 Notebook Running ABI 7300 Software
  • System Validation Report
  • Communication Cable
  • 120V Power Cord
  • User Manual
  • 30-Day Warranty

The ABI 7300 is in excellent condition and backed with our standard warranty.  The lamp on this real time PCR has less than one hour of use and system has been tested to meet factory specification.  In you have any questions about our Applied Biosystems 7300 system don't hesitate to give us a call.

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