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Amersham Biosciences AKTA Prime FPLC
Category:
Chromatography Manufacturer:
GE Lifesciences Listing #:
AMERSHAMEAKTAPRIMEFPLC-2426 Ships Within:
3 Business Days - EQUIPMENT DESCRIPTION
- SPECIFICATIONS
- INCLUDED
- NOTES
The Amersham Biosciences AKTA Prime FPLC offers a lab scale, one step, flexible solution for liquid chromatography and protein purification needs. It’s well suited to general purification tasks like gel filtration of recombinant DNA, ion exchange, buffer exchange and hydrophobic interaction chromatography, as well as affinity chromatography. With high reproducibility and accuracy, this compact FPLC includes a UV and conductivity monitors, and fraction collector and valves for buffer selection, flow diversion, and sample injection, as well as gradient formation. The Amersham Biosciences AKTA Prime FPLC comes pre-programmed with common use methods like sample cleanup, tagged protein affinity purification and MAbs, in simple to use formats.
The Amersham Biosciences AKTA Prime FPLC pump produces flow rates of up to 50 ml per minute with pressures up to 1MPa. Optimal results are achieved by using easy to attach pre-packed columns such as HiLoad, HiPrep or HiTrap. This protein purification system comes with AKTA PrimeView 5.31 software which allows the user to view the monitor signals from a run in real time. Results are automatical generated once a run is complete and saved in the log book. The PrimeView software records information such as curve data, run long, method information and system settings. User can save reports and convert to various file formats as well as print. This AKTA FPLC is great way for start-up labs to purify their own proteins and antibodies, instead of buying them from outsourced contract lab. Contact us today to learn more about our AKTA Prime FPLC.
Amersham Biosciences AKTAPrime FPLC Specifications:
Flow rate range | 0.1–50 ml/min in steps of 0.1 ml/min |
Pressure range | 0–1.0 MPa (10 bar, 145 psi) |
Pressure pulsation | < ±20% (dP/P) of mean value |
pH stability range | 1–14 (spec. valid between pH 2–12) |
Viscosity | < 5 ml/min > 5 ml/min Max. 10cP Max. 5 cP |
Flow rate reproducibility rsd | < 2% or 0.2 ml whichever is greater (at 0.1–50 ml/min) |
Flow rate accuracy | < ±4% or ±0.1 ml/min whichever is greater |
Gradient composition | Accuracy ±3% at 0.1–50 ml/min Reproducibility ±1.0% at 0.1–50 ml/min |
Leakage | < 1.0 µl/min (complete system) |
Pressure sensor | Range 0–1.0 MPa Scale error ±5% |
UV measurement | |
Wavelengths, Hg lamp, fixed | 254 and 280 nm |
Absorbance range | 0.01–5.0 AU |
Autozero range | -0.2–2.0 AU |
Baseline adjust Adjustable | 0–100% of full scale |
Linearity | < 3% up to 2 AU at 254 nm < 5% up to 1 AU at 280 nm |
Static noise | short term 40x10-6 AU at 254 nm long term 40x10-6 AU at 254 nm |
Static drift | ±100x10-6 AU/hour at 254 nm |
Flow sensitivity | 2x10-4 AU min/ml |
Flow rate | 0–100 ml/min |
Max. pressure | 4.0 MPa |
Max. back-pressure | 0.05 MPa at 100 ml/min |
Liquid temperature range | +4 to +60 °C |
Optical path length | 2 mm |
Cell volume | 2 µl (30 µl detector volume) |
Conductivity measurement | |
Conductivity range | 1 µS/cm to 999.9 mS/cm |
Reproducibility | short term Max. ±1% or ±5 µS/cm whichever is greater long term Max. ±3% or ±15 µS/cm whichever is greater |
Noise Max. | ±0.5% of full scale calibrated range |
Response time | < 3 s (0–95% of step) |
Temperature sensor | Accuracy ±2.0 °C Drift ±0.5 °C per 10 h |
Flow rate sensitivity | ±1% within 0–100 ml/min |
Conductivity flow cell | |
Flow rate | 0–100 ml/min |
Max. pressure | 5 MPa (50 bar, 725 psi) |
Max. back-pressure | 0.01 MPa at 100 ml/min |
Fraction collection | |
Tube capacity | 175 in tube rack 12 mm (optional) 95 in tube rack 18 mm 40 in tube rack 30 mm (optional) |
pH range | 0 to 14 (spec. valid between 2 and 12) |
Accuracy | temperature compensated ±0.1 pH within +4 to +40 °C not compensated ±0.2 pH within +15 to 25 °C, ±0.5 pH within +4 to +15 °C and +25 to +40 °C |
Response time | < 10 s (0–95% of step) |
Long term stability | Dev. max. 0.1 pH per 10 h at constant conditions (4–40 °C) |
Flow rate sensitivity | Dev. max. 0.1 pH units |
pH cell | |
Flow rate | 0.1–100 ml/min |
Max. pressure | 0.5 MPa (5 bar, 72 psi) |
Max. back-pressure | 0.02 MPa at 100 ml/min |
Dimensions | 18x14x21” |
Weight | 28lbs |
Power | 100–120/220–240 V ~, 50–60 Hz |
- GE AKTAprime FPLC System
- Windows 7 Laptop Running PrimeView 3.5.1
- Communication Cables
- Power Cords
- Validation Report with Clean Bill of Health
- Instruction Manual
- Original Packaging
- 30-Day Warranty
This listing is for a GE AKTA Prime Liquid Chromatography System. We've calibrated the instrument using the Acetone Trace Test to guarantee product performance, calibrated air pressure, and calibrated dispense pump. This FPLC system comes with current validations and is backed with our standard warranty. Should you have any questions about this or any of our other liquid chromotagraphs, please don't hesitate to contact us.
