Amersham Biosciences AKTA Prime FPLC

Category:

Chromatography

Manufacturer:

GE Lifesciences

Listing #:

AMERSHAMEAKTAPRIMEFPLC-2426

Ships Within:

3 Business Days

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EQUIPMENT DESCRIPTION
SPECIFICATIONS
INCLUDED
NOTES

The Amersham Biosciences AKTA Prime FPLC offers a lab scale, one step, flexible solution for liquid chromatography and protein purification needs. It’s well suited to general purification tasks like gel filtration of recombinant DNA, ion exchange, buffer exchange and hydrophobic interaction chromatography, as well as affinity chromatography. With high reproducibility and accuracy, this compact FPLC includes a UV and conductivity monitors, and fraction collector and valves for buffer selection, flow diversion, and sample injection, as well as gradient formation. The Amersham Biosciences AKTA Prime FPLC comes pre-programmed with common use methods like sample cleanup, tagged protein affinity purification and MAbs, in simple to use formats. Programs are accessible through a front panel LCD display.

The Amersham Biosciences AKTA Prime FPLC pump produces flow rates of up to 50 ml per minute with pressures up to 1MPa. Optimal results are achieved by using easy to attach pre-packed columns such as HiLoad, HiPrep or HiTrap. This protein purification system comes with AKTA PrimeView 5.31 software which allows the user to view the monitor signals from a run in real time. Results are automatical generated once a run is complete and saved in the log book. The PrimeView software records information such as curve data, run long, method information and system settings. User can save reports and convert to various file formats as well as print. This AKTA FPLC is great way for start-up labs to purify their own proteins and antibodies, instead of buying them from outsourced contract lab. Contact us today to learn more about our AKTA Prime FPLC.

Amersham Biosciences AKTAPrime FPLC Specifications:

Flow rate range	0.1–50 ml/min in steps of 0.1 ml/min
Pressure range	0–1.0 MPa (10 bar, 145 psi)
Pressure pulsation	< ±20% (dP/P) of mean value
pH stability range	1–14 (spec. valid between pH 2–12)
Viscosity	< 5 ml/min > 5 ml/min Max. 10cP Max. 5 cP
Flow rate reproducibility rsd	< 2% or 0.2 ml whichever is greater (at 0.1–50 ml/min)
Flow rate accuracy	< ±4% or ±0.1 ml/min whichever is greater
Gradient composition	Accuracy ±3% at 0.1–50 ml/min Reproducibility ±1.0% at 0.1–50 ml/min
Leakage	< 1.0 µl/min (complete system)
Pressure sensor	Range 0–1.0 MPa Scale error ±5%
UV measurement
Wavelengths, Hg lamp, fixed	254 and 280 nm
Absorbance range	0.01–5.0 AU
Autozero range	-0.2–2.0 AU
Baseline adjust Adjustable	0–100% of full scale
Linearity	< 3% up to 2 AU at 254 nm < 5% up to 1 AU at 280 nm
Static noise	short term 40x10-6 AU at 254 nm long term 40x10-6 AU at 254 nm
Static drift	±100x10-6 AU/hour at 254 nm
Flow sensitivity	2x10-4 AU min/ml
Flow rate	0–100 ml/min
Max. pressure	4.0 MPa
Max. back-pressure	0.05 MPa at 100 ml/min
Liquid temperature range	+4 to +60 °C
Optical path length	2 mm
Cell volume	2 µl (30 µl detector volume)
Conductivity measurement
Conductivity range	1 µS/cm to 999.9 mS/cm
Reproducibility	short term Max. ±1% or ±5 µS/cm whichever is greater long term Max. ±3% or ±15 µS/cm whichever is greater
Noise Max.	±0.5% of full scale calibrated range
Response time	< 3 s (0–95% of step)
Temperature sensor	Accuracy ±2.0 °C Drift ±0.5 °C per 10 h
Flow rate sensitivity	±1% within 0–100 ml/min
Conductivity flow cell
Flow rate	0–100 ml/min
Max. pressure	5 MPa (50 bar, 725 psi)
Max. back-pressure	0.01 MPa at 100 ml/min
Fraction collection
Tube capacity	175 in tube rack 12 mm (optional) 95 in tube rack 18 mm 40 in tube rack 30 mm (optional)
pH range	0 to 14 (spec. valid between 2 and 12)
Accuracy	temperature compensated ±0.1 pH within +4 to +40 °C not compensated ±0.2 pH within +15 to 25 °C, ±0.5 pH within +4 to +15 °C and +25 to +40 °C
Response time	< 10 s (0–95% of step)
Long term stability	Dev. max. 0.1 pH per 10 h at constant conditions (4–40 °C)
Flow rate sensitivity	Dev. max. 0.1 pH units
pH cell
Flow rate	0.1–100 ml/min
Max. pressure	0.5 MPa (5 bar, 72 psi)
Max. back-pressure	0.02 MPa at 100 ml/min
Dimensions	18x14x21”
Weight	28lbs
Power	100–120/220–240 V ~, 50–60 Hz

GE AKTAprime FPLC System
Windows 7 Laptop Running PrimeView 3.5.1
Communication Cables
Power Cords
Validation Report with Clean Bill of Health
Instruction Manual
Original Packaging
30-Day Warranty

This listing is for a GE AKTA Prime Liquid Chromatography System. We've calibrated the instrument using the Acetone Trace Test to guarantee product performance, calibrated air pressure, and calibrated dispense pump. This FPLC system comes with current validations and is backed with our standard warranty. Should you have any questions about this or any of our other liquid chromotagraphs, please don't hesitate to contact us.