BD FACSCalibur Flow Cytometry System with HTS Autosampler

Flow Cytometers
Becton Dickinson
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The Becton Dickinson FACSCalibur Flow Cytometry System is a compact, modular solution that accommodates both cell sorting and cell analysis. This easily upgraded system adapts to the evolving clinical and research requirements as well as new and emerging sorting and automation requirements. This flow cytometer utilizes standard multicolor analysis thanks to dual laser technology, interbeam compensation and alignment free optical design. Using a dual laser design for multi color analysis, the BD FACSCalibur features an air cooled argon laser and a red diode laser that are spatially separated.  

Optical path configuration to incorporates multiple fluorochromes has maximized the amount of information gained from a single sample making assays more cost effective. The Becton Dickinson FACSCalibur Flow Cytometry System is high performance, with superior sensitivity, maximum flexibility and minimal compensation. With its high speed analysis and multiparameter triggering system, the BD FACSCalibur flow cytometer is capable of rare-event detection like the characterization of subpopulation of stem cells. The BD FACSCalibur Flow Cytometry System is supported by a broad suite of user friendly software, designed to streamline a wide assortment of applications.

Applications such as enumeration of lymphocyte subsets, residual white blood cells, Reticulocytes, DNA analysis, immune function studies, stem cells, bead based immunoassays and multiplexed analysis of signal transduction and phosphorylation targets. It also offers a robust quality control system. The BD FACstation data management system is easy to use and automates many software functions, delivering fast acquisition and analysis tools for reporting, gating, statistical analysis and creating plots.  

Becton Dickson FACSCalibur Flow Cytometry System Specifications:

Lasers Included (2) Four Color Detection488nm argon ion laser and 635nm red diode, both are air cooled
Forward Scatter and Side Scatter SensitivitySensitivity activates separation of platlets from background noise
Forward and Side Scatter Resolutionenhanced for determining monocytes, granulocytes and lymphocytes
Flourescence Sensitivity Detection up to 200 FITC which is equal to molecules per particle
Fluorescence ResolutionConstant variations in the FL2 Area
Cell Sorting FeatureYES
Purifity of Cell SortingLess Than 95% 
Cell Sorting ModesSingle Cell, Recovery and Exclusion
Cell Sorting Ratesup to 300 cells per second
Power 120V 50/60Hz
Dimensions (W x D x H)35" x 26" x 27"
Weight250 lb

  • Becton Dickinson FACSCalibur (2 Laser / 4 Color ) Flow Cytometer
  • MAC OSX Computer Running CellQuest Pro with USB License Dongle
  • HTS Microplate Autosampler
  • Single Sample Cell Loader
  • Operator's Manual
  • Power Cables
  • Communication Cables
  • Calibration Report
  • Warranty

This BD FACSCalibur Flow Cytometer comes fully calibrated to factory specification.  We use a bead concentration kit to check all of our Flow Cytometry systems prior to shipping.  System includes the BD High Throughput Autosampler, but we also have spare sample loaders in stock if needed.  If you have any questions about our used flow cytometers don't hesitate to give us a call.  

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