Miltenyi Biotec autoMACS Pro Separator

Flow Cytometers
Miltenyi Biotec
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The Miltenyi Biotec autoMacs Pro is a powerful automated cell separator used to isolate cells from whole blood, or bone marrow.  This cell separator is ideal for researchers with myeloma research along with analysis for lineage specific chimersim required for bone marrow transplantation.  The Miltenyi Biotec autoMacs Pro works with a variety of Cell Separation Reagents used for quick isolation of any cell type from any known species.  Cell seperation has become extremely popular among flow cytometry labs in the last decade and the autoMACS Pro is now the gold standard in fluorescence activated cell sorting.  This system uses a unique MicroBead technology which allows the instrument to be also identified as a flow sorter.  The Miltenyi Biotec autoMACS Pro Separator can be used with a number of applications including cell culture, flow cytometry, FISH, PCR, and much more. 

This easy to use system makes sorting cells a seamless three step process and can sort more than ten million cells per second.  Just scan your reagents with the autoMACS Pro Separator barcode reader from Miltenyi Biotec, position the samples along with the reagents on to the cell sorter, select the run button on the LCD controller and walk away.  It’s that easy!  The Miltenyi Biotec barcode reader has the ability to identify each reagent and will choose the correct separation protocol and wash program needed for you analysis.  This hands free method ensures safer lab practices which are key for handling hazardous samples.

The Miltenyi Biotec autoMACS Pro automated cell sorter includes twelve pre-programmed separation protocols for reproducible cell separation.  The cell isolation program is chosen based on the target cell frequency, cell separation strategy and level of antigen expression.  The built-in LCD touchscreen controller provides straightforward operation and continuous monitoring of sample processing.  The Miltenyi Biotec autoMACS Pro Separator is extremely easy to maintain and comes with built in features including automated start-up, cleaning and shutdown, along with optimized washing and decontamination maintenance programs.   After each cell separation the automated cell sorter performs an in-depth washing protocol and then refills the column with running buffer.   

The Miltenyi Biotec autoMACS Pro separator comes with automated sensor controlled procedures which guarantee absolute safety of your samples.  The illumination of bottles reflect the status of the cell sorter, while constantly policing buffer levels and column status.  The system will maintain your samples at 4°C throughout separation and labeling process by using the Miltenyi Biotec MACS chill racks.  By doing away with temperature deviations you can ensure higher cell viability rates and dodge alterations in gene expression.  The autoMACS Pro cell separator can process up to six samples in one batch including 5ml, 15ml, and 50ml tubes. 

The Miltenyi Biotec autoMACS Pro Separator uses MACS MicroBeads which are 50nm sized.  Theses super paramagnetic particles conjugated to specific antibodies which target an antigen on the cell surface.  The unique technology is ideal for cell sorter and flow cytometry applications as the tiny size of each microbead will not saturate the cell surface epitopes.  Unlike large beads used with BD FACSCalibur, BD FACSCanto, or Becton Dickinson FACSAria systems, the autoMACS Pro cell separator does not require removal of the microbeads before use with downstream applications such as FISH, flow cytometry and PCR.  This is extremely useful when isolating cells from whole blood, or bone marrow because it allows you to avoid cell lysis needed for red blood cells, eliminating the gradient centrifugation process which is typically required in flow cytometry.  

The Whole Blood MicroBeads are quickly added to blood samples and treated with anticoagulant; leukocyte subsets which get purified with this automated cell sorter.  The process minimizes cell loss ideal for small blood volumes and purifies cells which works well for molecular biology research, flow cytometry analysis, and other functional studies.  Contact us today to learn more about our Miltenyi Biotec autoMACS Pro Separator.

Miltenyi Biotec autoMACS Pro Separator Specifications: 

Sample Tube Sizes

5ml, 15ml, 50ml can be used


12 Pre-Sets


100-240 VAC 50/60Hz

Dimensions (W x D x H)   

23" x 14" x 16" 


58 lbs                                                                                                                                                    

  • Miltenyi Biotec autoMACS Pro Separator (Magnetic Cell Sorter) # 130-092-545
  • Validation Report with Calibration and Clean Bill of Health
  • Buffer, Waste, Rinse and Storage Bottles
  • Touch Screen Controller with Built-In Software
  • Column
  • 15ml Chill Rack or a 5 and 50 mL chill rack pacakge
  • Operator's Guide
  • Power Cable
  • Tubing
  • 30-Day Warranty

This Miltenyi Biotec autoMACS Pro Separator has been thoroughly inspected and comes with a clean bill of health and current validations. Please indicate which chill rack pacakge you'd prefer , 15 mL, or a 5 and 50 mL option. All of our lab equipment comes backed with our standard warranty.  Should you have any used flow cytometers for sale please let us know.  We're always looking for good cytometers such as BD FACSCanto, BD FACSAria, BD Accuri C6, FACSCaliburs, Millipore Guava EasyCyte Pro and of course the Miltenyi AutoMacs and Miltenyi MACSQuant systems