Molecular Devices SpectraMax M5 Plate Reader

Microplate Readers
Molecular Devices
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The Molecular Devices Spectramax M5 Multi-Mode Microplate Reader brings you the power and precision of a dedicated single mode plate reader, but with the space saving flexibility of 5 detection modes in one unit. This microplate reader handles Absorbance, Fluorescence Intensity, luminescence, Time resolved fluorescence, fluorescence polarization, HTRF, and comes with 140 preprogrammed protocols to get you up and running fast. This microplate reader has a wavelength range of 200–1000 nm, uses two monochromators, allowing users to choose their optimal emission or excitation and eliminating the need to change filters. The SpectraMax M5 covers formats from cuvettes to 6-, 12-, 24-, 48-, 96-, and 384-well microplates and incorporates a triple-mode cuvette port, accurate temperature control, and microplate shaking. 

The Molecular Devices SpectraMax M5 Multi-Mode Plate Reader uses a dedicated photomultiplier tube to reduce stray light and boost the best signal to noise. For luminescence reading by using Instead of lenses this Plate Reader uses elliptical mirrors that maximize transmission, while minimizing wavelength distortion. The Spectramax M5 utilizes powerful PathCheck Sensory technology to measure the depth of samples without being temperature dependent. In addition, when used with Soft Max Pro, the Path Check Sensor of this microplate reader will normalize the well absorbance, letting users calculate directly without absorptive compounds. This microplate is robot compatible as well to help boost productivity and automation and features customizable spreadsheets and powerful graphing tools.

Molecular Devices SpectraMax M5 Specifications:



Plate formats

6, 12, 24, 48, 96, 384 wells

Light source

Xenon Flash Lamp (1 joule/flash)


2 photomultiplier tubes (PMT)

Shaker time

0 to 999 seconds

Temp. control

2°C above ambient to 60°C

Temp. uniformity

< 1°C at 37°C set point

Temp. accuracy

±1°C at 37°C set point


Endpoint, Kinetic Spectral Scanning

Standard Read Times (minutes:seconds)* 96 wells 384 wells


Absorbance 0:18 0:49

Fluorescence Intensity 0:17 0:48

Luminescence 2:00 7:00

Absorbance Photometric Performance


Wavelength range

200–1000 nm

Wavelength selection

Monochromator, tunable in 1.0 nm increments

Wavelength bandwidth

≤ 4.0 nm

Wavelength accuracy

±2.0 nm

Wavelength repeatability

±0.2 nm

Photometric range

0–4.0 OD

Photometric resolution

0.001 OD

Photometric accuracy

(microplate) < ±0.006 OD ±1.0%, 0–2 OD

 (cuvette) < ±0.005 OD ±1.0%, 0–2 OD


Photometric precision

< ±0.003 OD ±1.0%, 0–2 OD

Stray light

< 0.05% @ 230 nm

Fluorescence Intensity Performance

Top or bottom

Wavelength range

250–850 nm

Wavelength selection

Monochromators, tunable in 1.0 nm increments

Bandwidth (EX, EM)

9 nm, 15 nm

Optimized sensitivity

≤ 1 pM fluorescein in 96 wells,

≤ 1.5pM in 384 wells

Guaranteed sensitivity

< 5 pM fluorescein in 96 wells or cuvette,

 < 20 pM in 384 wells

Luminescence Performance

Top or Bottom

Wavelength selection

Choice of simultaneous detection of all wavelengths or selection via monochromator, tunable in 1.0 nm increments

Wavelength range

250–850 nm

Optimized sensitivity

≤ 43pM ATP in 96 wells

Guaranteed sensitivity 

≤ 75pM ATP in 96 wells

Dynamic range

> 6 decades


< 0.3% in white 96- and 384-well Microplates


23 x 16 x 9 inches


36 lbs


< 420 watts, 100–240 VAC, 3.5 A, 50/60Hz

Time Resolved Fluorecesnce Performance

Top or Bottom of a Microplate

Wavelength selection Monochromators,

tunable in 1.0 nm increments

Bandwidth (EX, EM)

9 nm, 15 nm

Precision data collection

1–100 flashes, delay of 0–600 µsec. before read, integration time selectable between 50–1500 µsec.

Optimized sensitivity

≤ 10 fM europium in 96

Guaranteed sensitivity

≤ 100 fM europium in 96 or 384 wells

SpectraMax M5 Reader only

Certified to Cisbio Bioassays HTRF technology performance specifications

Fluorescence Polarization Performance


Wavelength range

300–750 nm

Wavelength selection

Monochromators, tunable in 1.0 nm increments

Bandwidth (EX, EM)

9 nm, 15 nm

Optimized Precision

≤ 3.5 mP standard deviation at 1 nM fluorescein in 96 wells

Guaranteed Precision

< 5 mP standard deviation at 1 nM fluorescein in 96 wells




  • Molecular Devices Spectramax M5 Microplate Reader 
  • Windows 7 Notebook with SoftMax Pro 6
  • Absorbance Validation Report
  • Fluorescence Validation Report
  • Communication Cable
  • 120V Power Cord
  • Operator's Guide
  • 30-Day Warranty

This Molecular Devices SpectraMax M5 Multi-Mode Plate Reader has been fully tested and validated by our service team with a clean bill of health.  Instrument includes both UV-Vis and Fluorescence calibration reports and plate readers ships out via freight.

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