Thermo Evolution 300 UV-Vis Spectrophotometer

Thermo Scientific
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The Thermo Scientific Evolution 300 is a high performance uv-vis spectrophotometer offering a wide variety of applications designed for pharmaceutical, biochemistry and life sciences research.  The Thermo Evolution 300 UV-Vis Spectrophotometer is controlled by VisionSecurity software which includes an easy to use automated wizard to assist the user with setting up and customizing assays.  Some of the more popular assays used with this Spectrophotometer include Nucleic Acid Test, Kinetics, Protein Concentration, Thermal Denaturation, Thermo Renaturation, Quantitative Analysis and more. 

The Thermo Evolution 300 UV-Vis Spectrophotometer uses a powerful pulsing Xenon lamp along with a variable bandwidth platform making it one of the most sensitive absorbance spectrophotometers on today’s market.  This instrument works well with QA and QC applications along with 5 variable spectral bandwidth settings.  This robust spectrophotometer has an acquisition rate of 50 data points per second and is ready to use out of the gates.   No more waiting to warm the system up before use.  The Evolution series VisionSecurity software accompany this system is 21 CFR Part 11 compliance allowing to submit data for audit trail.  The Thermo Evolution 300 UV-Vis Spectrophotometer has everything you need to bring your research to life.  Contact us today to learn more about our used lab instruments.

Thermo Evolution 300 UV/Vis Spectrophotometer Specifications:


±0.004A at 1A, ±0.004A at 2A, ±0.006A at 3AÅ

Accuracy (Photometric)    

±0.004A at 1A, ± 0.004A at 2A, ± 0.006A at 3A

Baseline Flatness       

±0.0015A (200-800nm), 2.0nm SBW, smoothed


<0.0005A/hr, 500nm, 2.0nm SBW, 2hr warm-up


Computer Control



Min. Data Interval    


Scan Speed   

1 to 3800nm/min

Spectral Bandwidth

0.5; 1.0; 1.5; 2.0; 4.0nm

Stray Light  

KCl, 198nm: >2.0A , NaI, 220nm: >3.7A , NaNO2 340nm: >3.9A

Wavelength Accuracy   

±0.20nm (546.11nm Hg emission line); ±0.3nm for 190-900nm

Wavelength Range

190 to 1100nm

Wavelength Repeatability

<0.05nm (standard deviation of 10 measurements), < 0.10nm (peak separation of repetitive scanning of a Hg line source) 

  • Thermo Scientific Evolution 300 UV/Vis Spectrophotometer
  • Windows 7 Notebook with Software
  • Sample Cell Holder
  • Operator's Guide
  • Communication Cable
  • Warranty

This used lab equipment listing is for a fully calibrated Thermo Evolution 300 UV-Vis Spectrophotometer.  We calibrated the spectrophotometer using our own house standards purchased from Thermo Scientific.  The instrument comes with a calibration report showcasing the systems performance and is backed by our standard warranty.  Should you have any questions, please don't hesitate to contact us.

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