Waters Acquity UPLC System

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The Waters Acquity UPLC system provides you with reproducible, dependable and data rich results for challenging separations. This UPLC system features a wide range of flow rates, back pressures and linear velocities, with increased throughput with high efficiency so you get the most for your time and resources. This contained tower uplc system holds a sample manager, column heater, binary solvent manager, and a PDA detector. This ultra-performance liquid chromatography system has a unique sub 2 μm particle system that offers 3 times the efficiency of a 5 μm system with 70% higher resolution. This higher resolution means more information from more samples in less time. The Waters UPLC sytem can then run separations at a flow rate 3 times faster than HPLC, without a loss in resolution. This Aquity system contains a PDA Detector with a brand new lamp, sampler manager autosampler, a binary solvent manager, as well as a solvent and waste tray. 

The Waters Acquity UPLC system easily converts HPLC methods to UPLC thanks to the UPLC console calculator built to support existing HPLC Methods and coordinate with third party MS solutions such as those offered by Sciex, Bruker Daltonics and Thermo Fisher.  Data acquired by the Acquity system is managed, analyzed and stored by Empower Software, a user friendly graphic icon based interface, run on a windows PC. This powerful software allows users to see results in real time, with a summary of previous data collected.  This ultra-performance LC system can sample up to 80 data points per second, of for the fast scan a sample rate of 25-50 points is recommended. The Acquity UPLC can store up to 4 solvents and has a settable flow rate of 0.001 to 2.000 mL/min, in 0.001 mL increments. This Acquity UPLC comes with a photodiode array detector with a brand new lamp and a wavelength range of 190 to 500 nm, and a column heater that can accommodate waters columns up to 4.6 mm ID and 150mm long with a temperature range of 5°C above ambient to 65°C.

Waters Acquity UPLC System Specifications:

Solvent Manager


Number of solvents

Up to four, in any combination of two:

A1 or A2 and B1 or B2.

Solvent conditioning

Vacuum degassing (six-channel): one channel per solvent, and two channels for sample manager wash solvents.

Settable flow rate range

0.001 to 2.000 mL/min, in 0.001 mL increments

Gradient profiles

Eleven gradient curves [including linear, step (2), concave (4), and convex(4)] Wet prime Automatic


Maximum operating pressure

103,421 kPa (1034 bar, 15,000 psi) up to 1  L/min, 62,053 kPa (621 bar, 9000 psi) up to 2 mL/min

Composition accuracy

±0.5% absolute (full scale) from 5 to 95% from 0.50 to 2.00 mL/mina

Composition precision

0.15% RSD or ±0.04 min SD

Flow accuracy

±1.0% at 0.5 mL/min

Sample Manager


Number of SBS/ANSI sample plates

Total of two plates, expandable to up to 22 plates with optional sample organizer.

 Maximum of 22 microtiter plates, 11 intermediate depth plates, or 8 deep-well (or 2 mL vial) plates:

96 and 384 microtiter plates

48-position 2 mL vial plates

48-position 0.65 mL micro-centrifuge tube plates

24-position 1.5 mL micro-centrifuge tube plates

Maximum sample capacity

768 in two 384-well plates; expandable to up to 8,448 samples with optional sample organizer

Injection volume range

0.1 to 50 µL, in 0.1 µL increments,

partial or full loop mode, 10 µL loop is

standard; 1, 2, 5, 20, and 50 µL loops


Sample temperature control

4 to 40 °C, programmable in 0.1 °C increments

Minimum sample required

5 µL residual volume at a needle offset of 0, or 22 µL at the preset 2 mm needle offset

Column Heater 


Columns accepted

Any up to 4.6 mm ID and 150mm long

Temperature range

5 above ambient to 65°C (9 above ambient to 194°F)

PDA Detector


Wavelength Range

190-500 nm

Flow Cell Options

Analytical Cell 500 nanoliters, 10 mm pathlength

High Sensitivity Cell 2.4 microliters, 25mm pathlength


25 x 14 x 34”


100-240v, 50/60 Hz,

  • Waters Acquity UPLC system
  • Waters Empowers 2 Computer Workstation
  • PDA Detector with Brand New Lamp Installed
  • Sampler Manager Autosampler
  • Binary Solvent Manager
  • Solvent and Waste Tray
  • Communication Cables
  • Operator's Guide
  • 115V Power Cords
  • 30-Day Warranty
  • System received a full PM with clean bill of health

This Waters Acquity UPLC system has been fully inspected and comes with a brand new lamp. When setting up this UPLC system, be sure to avoid positioning under an HVAC vent, and due to the weight, the use of two people to lift is recommended. Also to make sure there is proper ventilation of the Aquity UPLC system, allow a 6 inch clearance in the back.  All of our lab equipment comes backed with our standard warranty. Should you have any questions, please let us know, we're here to help.

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