Agilent 1100 HPLC with VWD

G1314A VWD Detector

Detection type

Double-beam photometer

Light source

Deuterium lamp

Wavelength range

190–600 nm

Short term noise (ASTM)

± 0.75 × 10-5 AU at 254 nm

Drift

3 × 10-4 AU/hr at 254 nm

Linearity

> 2 AU (5%) upper limit

Wavelength accuracy

± 1 nm Self-calibration with deuterium lines, verification with holmium oxide filter

Bandwidth

6.5 nm typical

Flow cells

Standard: 14-µl volume, 10-mm cell path length and 40 bar (588 psi) pressure maximum High pressure: 14-µl volume, 10-mm cell path length and 400 bar (5880 psi) pressure maximum Micro: 1-µl volume, 5-mm cell path length and 40 bar (588 psi) pressure maximum Semimicro: 5-µl volume, 6-mm cell path length and 40 bar (588 psi) pressure maximum

Analog outputs

Recorder/integrator: 100 mV or 1 V, output range 0.001 – 2 AU, one output

Agilent G1316A Colcom Thermostated Column Compartment Specifications:

Temperature Range

10° below ambient to 80° C

Temperature Stability

± 0.15°C

Temperature Accuracy

± 0.8°C

Column Capacity

Three 30cm columns

Warm Up/Cool Down

5 mins from ambient to 40°C

Dead Volume

3 µl left heat exchanger

6 µl right heat exchanger

G1329A Auto Sampler

Metering device

Metering pump in high-pressure flow

Injection range

 0.1 – 100 µl in 0.1 µl increments

Replicate injections

1 – 99 from one vial

Precision

< 0.25% RSD from 5 – 100 µl, < 1% RSD 1 – 5 µl variable volume

Minimum sample volume

1 µl from 5 µl sample in 100 µl microvial, or 1 µl from 10 µl sample in 300 µl microvial

Carryover Typically

< 0.1%, < 0.05% with external needle cleaning

Sample viscosity range

0.2 – 50 cp

Sample capacity

100 × 2-ml vials in 1 tray 40 × 2-ml vials in ½ tray 15 × 6-ml vials in ½ tray Agilent vials only)

Injection cycle time

Typically 50 s depending on draw speed and injection volume

G1312A Binary Pump

Setable flow range

Setpoints 0.001 – 5 ml/min, in 0.001 ml/min increments

Flow range

0.1 – 5.0 ml/min

Flow accuracy

± 1% or 10 µl/min whatever is greater

Pressure Operating range

 0 400 bar (0 – 5880 psi) up to 5 ml/min

Pressure pulsation

< 2 % amplitude (typically < 1 %), at 1 ml/min isopropanol, at all pressures > 1 MPa

Compressibility compensation

User-selectable, based on mobile phase compressibility

Recommended pH

range 1.0 – 12.5, solvents with pH < 2.3 should not contain acids which attack stainless steel

Gradient formation

High-pressure binary mixing, delay volume 180 – 480 µl without a mixer, 600 – 900 µl with mixer, dependent on back pressure

Vacuum Degasser G1379A

Composition range

 1 – 99 % or 5 µl/min per channel, whatever is greater

Setable flow range

Setpoints 0.001 – 5 ml/min, in 0.001 ml/min increments

Flow range

0.1 – 5.0 ml/min

Flow accuracy

± 1% or 10 µl/min whatever is greater

Pressure Operating range

 0 400 bar (0 – 5880 psi) up to 5 ml/min

Pressure pulsation

< 2 % amplitude (typically < 1 %), at 1 ml/min isopropanol, at all pressures > 1 MPa

Compressibility compensation

User-selectable, based on mobile phase compressibility

Recommended pH

range 1.0 – 12.5, solvents with pH < 2.3 should not contain acids which attack stainless steel

Gradient formation

High-pressure binary mixing, delay volume 180 – 480 µl without a mixer, 600 – 900 µl with mixer, dependent on back pressure

Composition range

 1 – 99 % or 5 µl/min per channel, whatever is greater

Composition precision

0.5% absolute

Agilent G1330B ALS Thermostat

Temperature range

4 °C to 40 °C in 1 ° increments

Temperature accuracy at ambient temperatures < 25 °C and humidity < 50 %

3 °C to 8 °C at a setpoint of 4 °C*

Temperature accuracy at ambient temperatures <30 °C and humidity <60 %

3 °C to 9 °C at a setpoint of 4 °C*