Agilent 1100 HPLC with VWD
Chromatography
Agilent
Chromatography Price
- EQUIPMENT DESCRIPTION
- SPECIFICATIONS
- INCLUDED
- NOTES
The Agilent 1100 HPLC offers you a customizable and highly accurate chromatography system for HPLC analysis. The Agilent series is robust and versatile with each component operating seamlessly in concert with the others or as standalone instruments controlled by a PC. This HPLC stack comes configured with a Variable Wavelength detector, Colcom, Autosampler, a Low pulse binary pump, and a micro vacuum degasser. The Agilent G1312A Binary Pump provides high-pressure mixing and great gradient generation with a two-pump assembly for the Agilent 1100 HPLC, that work together, metering solvent and delivering to the high-pressure side, generating up to 400 Barr in pressure.
The Agilent 1100 Series G1314A VWD is a high-performance Variable Wavelength Detector, that can be used independently or added as a component to an 1100 HPLC system. This VWD employs a deuterium lamp that provides high intensity and a low detection limit, with a wavelength range of 190-600 nm. The G1316A Colcom is a temperature-controlled column compartment built to handle Peltier heating and cooling and holds up to three 30cm columns. The Agilent G1379A Vacuum Degasser also allows users to incorporate smaller diameter columns effectively by creating a stable low flow rate through a 4-channel container, 4 tube membranes and a vacuum pump. Data acquired by this Chromatography system can is then processed with the controlling Chemstation software, with configurable reports and in-depth analysis including integration, quantification and reporting, and it also allows you to automate and customize your whole instrument use.
G1314A VWD Detector | |
Detection type | Double-beam photometer |
Light source | Deuterium lamp |
Wavelength range | 190–600 nm |
Short term noise (ASTM) | ± 0.75 × 10-5 AU at 254 nm |
Drift | 3 × 10-4 AU/hr at 254 nm |
Linearity | > 2 AU (5%) upper limit |
Wavelength accuracy | ± 1 nm Self-calibration with deuterium lines, verification with holmium oxide filter |
Bandwidth | 6.5 nm typical |
Flow cells | Standard: 14-µl volume, 10-mm cell path length and 40 bar (588 psi) pressure maximum High pressure: 14-µl volume, 10-mm cell path length and 400 bar (5880 psi) pressure maximum Micro: 1-µl volume, 5-mm cell path length and 40 bar (588 psi) pressure maximum Semimicro: 5-µl volume, 6-mm cell path length and 40 bar (588 psi) pressure maximum |
Analog outputs | Recorder/integrator: 100 mV or 1 V, output range 0.001 – 2 AU, one output |
Agilent G1316A Colcom Thermostated Column Compartment Specifications: | |
Temperature Range | 10° below ambient to 80° C |
Temperature Stability | ± 0.15°C |
Temperature Accuracy | ± 0.8°C |
Column Capacity | Three 30cm columns |
Warm Up/Cool Down | 5 mins from ambient to 40°C |
Dead Volume | 3 µl left heat exchanger 6 µl right heat exchanger |
G1329A Auto Sampler | |
Metering device | Metering pump in high-pressure flow |
Injection range | 0.1 – 100 µl in 0.1 µl increments |
Replicate injections | 1 – 99 from one vial |
Precision | < 0.25% RSD from 5 – 100 µl, < 1% RSD 1 – 5 µl variable volume |
Minimum sample volume | 1 µl from 5 µl sample in 100 µl microvial, or 1 µl from 10 µl sample in 300 µl microvial |
Carryover Typically | < 0.1%, < 0.05% with external needle cleaning |
Sample viscosity range | 0.2 – 50 cp |
Sample capacity | 100 × 2-ml vials in 1 tray 40 × 2-ml vials in ½ tray 15 × 6-ml vials in ½ tray Agilent vials only) |
Injection cycle time | Typically 50 s depending on draw speed and injection volume |
G1312A Binary Pump | |
Setable flow range | Setpoints 0.001 – 5 ml/min, in 0.001 ml/min increments |
Flow range | 0.1 – 5.0 ml/min |
Flow accuracy | ± 1% or 10 µl/min whatever is greater |
Pressure Operating range | 0 400 bar (0 – 5880 psi) up to 5 ml/min |
Pressure pulsation | < 2 % amplitude (typically < 1 %), at 1 ml/min isopropanol, at all pressures > 1 MPa |
Compressibility compensation | User-selectable, based on mobile phase compressibility |
Recommended pH | range 1.0 – 12.5, solvents with pH < 2.3 should not contain acids which attack stainless steel |
Gradient formation | High-pressure binary mixing, delay volume 180 – 480 µl without a mixer, 600 – 900 µl with mixer, dependent on back pressure |
Vacuum Degasser G1379A | |
Composition range | 1 – 99 % or 5 µl/min per channel, whatever is greater |
Setable flow range | Setpoints 0.001 – 5 ml/min, in 0.001 ml/min increments |
Flow range | 0.1 – 5.0 ml/min |
Flow accuracy | ± 1% or 10 µl/min whatever is greater |
Pressure Operating range | 0 400 bar (0 – 5880 psi) up to 5 ml/min |
Pressure pulsation | < 2 % amplitude (typically < 1 %), at 1 ml/min isopropanol, at all pressures > 1 MPa |
Compressibility compensation | User-selectable, based on mobile phase compressibility |
Recommended pH | range 1.0 – 12.5, solvents with pH < 2.3 should not contain acids which attack stainless steel |
Gradient formation | High-pressure binary mixing, delay volume 180 – 480 µl without a mixer, 600 – 900 µl with mixer, dependent on back pressure |
Composition range | 1 – 99 % or 5 µl/min per channel, whatever is greater |
Composition precision | 0.5% absolute |
Agilent G1330B ALS Thermostat |
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Temperature range | 4 °C to 40 °C in 1 ° increments |
Temperature accuracy at ambient temperatures < 25 °C and humidity < 50 % | 3 °C to 8 °C at a setpoint of 4 °C* |
Temperature accuracy at ambient temperatures <30 °C and humidity <60 % | 3 °C to 9 °C at a setpoint of 4 °C* |
- Agilent 1100 Series HPLC System
- Laptop Running LabAdvisor and Chemstation Software
- Validation Reports for Each Agilent 1100 Module
- G1314A VWD Variable Wavelength Detector
- G1329A Autosampler
- G1330B Thermostat for Autosampler
- G1316A Thermostat Column Compartment
- G1379A Micro Degasser
- G1312A Binary Pump
- CAN Communication Cables
- 120V Power Cords
- Agilent 1100 User Manual
- 30-Day Warranty
- Please Note: system has been checked out by our service team with a clean bill of health. We will provide validation reports for each module, if you would like to see them in advance, please don't hesitate to contact us. We've included a couple of the reports on the pictures above, but there are over 15 reports we have on file for this system.
This Agilent 1100 HPLC has been fully inspected and comes with validation reports for every component. All of our lab equipment comes backed with our standard warranty. Should you have any questions about this or any of our Chromatography Equipment, please don't hesitate to contact us.