Agilent 1100 HPLC with DAD

DAD Detector

Detection type

1024-element photodiode array

Light source

Deuterium and tungsten lamps

Data Rate

Up to 80 Hz

Wavelength range

190 – 950 nm

ASTM single & multi-wavelength

± 1° x 10-5 AU at 254 and 750 nm

Drift

2° x 10-3 AU / hr at 254 nm

Linear absorbance range

> 2 AU (upper limit)

Wavelength accuracy

± 1 nm Self-calibration with deuterium lines, verification with holmium oxide filter

Wavelength bunching

1 – 400 nm Programmable in steps of 1 nm

Slit width

1, 2, 4 , 8, 16 nm Programmable slit

Diode width

 < 1 nm

Flow cell Standard

Standard :13-µL volume, 10 mm cell path length, 120 bar (1760 psi) pressure maximum Micro: 2µl volume, 3mm cell path length and 120 bar (1760 psi) pressue maximum

Semi Micro: 5µl volume. 6 mm cell path length and 120 (1760 psi) pressure maximum

Temperature Range

10° below ambient to 80° C

Temperature Stability

± 0.15°C

Temperature Accuracy

± 0.8°C

Column Capacity

Three 30cm columns

Warm Up/Cool Down

5 mins from ambient to 40°C

Dead Volume

3 µl left heat exchanger

6 µl right heat exchanger

ALS Auto Sampler

Injection range

 0.1 – 100 µl in 0.1 µl increments

Replicate injections

1 – 99 from one vial

Precision

< 0.25% RSD from 5 – 100 µl, < 1% RSD 1 – 5 µl variable volume

Minimum sample volume

1 µl from 5 µl sample in 100 µl microvial, or 1 µl from 10 µl sample in 300 µl microvial

Carryover Typically

< 0.1%, < 0.05% with external needle cleaning

Sample viscosity range

0.2 – 50 cp

Replicate injections per vial

1 – 99

Sample capacity

100 × 2-ml vials in 1 tray 40 × 2-ml vials in ½ tray 15 × 6-ml vials in ½ tray Agilent vials only)

Injection cycle time

Typically 50 s depending on draw speed and injection volume

Binary  Pump

Setable flow range

Setpoints 0.001 – 5 ml/min, in 0.001 ml/min increments

Flow range

0.1 – 5.0 ml/min

Flow accuracy

± 1% or 10 µl/min whatever is greater

Pressure Operating range

 0 400 bar (0 – 5880 psi) up to 5 ml/min

Pressure pulsation

< 2 % amplitude (typically < 1 %), at 1 ml/min isopropanol, at all pressures > 1 MPa

Compressibility compensation

User-selectable, based on mobile phase compressibility

Recommended pH

range 1.0 – 12.5, solvents with pH < 2.3 should not contain acids which attack stainless steel

Gradient formation

High-pressure binary mixing, delay volume 180 – 480 µl without mixer, 600 – 900 µl with mixer, dependent on back pressure

Degasser G1379A

Composition range

 1 – 99 % or 5 µl/min per channel, whatever is greater

Setable flow range

Setpoints 0.001 – 5 ml/min, in 0.001 ml/min increments

Flow range

0.1 – 5.0 ml/min

Flow accuracy

± 1% or 10 µl/min whatever is greater

Pressure Operating range

 0 400 bar (0 – 5880 psi) up to 5 ml/min

Pressure pulsation

< 2 % amplitude (typically < 1 %), at 1 ml/min isopropanol, at all pressures > 1 MPa

Compressibility compensation

User-selectable, based on mobile phase compressibility

Recommended pH

range 1.0 – 12.5, solvents with pH < 2.3 should not contain acids which attack stainless steel

Gradient formation

High-pressure binary mixing, delay volume 180 – 480 µl without mixer, 600 – 900 µl with mixer, dependent on back pressure

Composition range

 1 – 99 % or 5 µl/min per channel, whatever is greater

Composition precision

0.5% absolute