Agilent 1100 Series LC System

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The Agilent 1100 Series LC System provides you with in depth molecular information in a modular instrument that’s easy to maintain and intuitive to use. This Capillary LC system brings you a complete analytical solution, with fully integrated hardware and software to boost your productivity and give you results of which you can be confident. This Liquid Chromatography System consists of a DAD Detector, binary pump, thermostated column compartment, autosampler, fraction collector with thermostat and a vacuum degasser. The Agilent 1100 Series LC lets you analyze a broad spectrum of compounds such as proteins and peptides, pesticides and herbicides, as well as drugs and drug metabolites. This LC system helps shorten analysis times, while providing robust information such as molecular weight, structure, low level impurities, and quantitative information without the need for fully resolved peaks, letting you do more in a single run with less preparation.


The Agilent 1100 Series LC lets you use existing HPLC methods through an orthogonal-spray ion source and high-capacity drying gas system for exceptional sensitivity and reproducibility. The benefits of the orthogonal spray of this Capillary LC system are reduced background and ion source contamination, optimized desolvation and ion transport, and it eliminates the need to reposition for changes to flow rate or solvent compositions. The Agilent 1100 Series LC also comes equipped with Chemstation Software, to guide users through a graphical interface through automated features to get you up and running fast. This powerful software is Windows based and allows you to control both data acquisition and set parameters all from only two windows. UV and Mass Spectral data from this LC system can be processed together to help speed up your processes. The 1100 series is also simple to maintain thanks to the built-in diagnostics, integrated validation tools and remote access maximize uptime and make maintenance easier.

 Agilent 1100 Series LC System Specifications:

Mass Range


VL – m/z 50 - 1500

SL – m/z 50 - 3000

Mass accuracy

± 0.13 u within the calibrated mass range in scan mode

Mass axis stability

± 0.13 u over 8 hours

SIM sensitivity

API-electrospray at 400 µl/min or APCI at 1000 µl/min

Selected ion monitoring of m/z 609.3

Positive ionization

Scan sensitivity

API-electrospray at 400 µl/min or APCI at 1000 µl/min

Scan range m/z 100 – 650

Extracted ion at m/z 609.3

Positive ionization

DAD Detector


Detection type

1024-element photodiode array

Light source

Deuterium and tungsten lamps

Wavelength range

190 – 950 nm

Short term noise (ASTM) Single and Multi-Wavelength

Typically ± 3 × 10-5 AU at 254 nm at flow rates 100 µl/min For the 500 nl flow cell the noise is 2-3 times higher than with standard flow cell


2 × 10-3 AU/hr at 254 nm

Linear absorbance range

> 2 AU (upper limit)

Wavelength accuracy

± 1 nm Self-calibration with deuterium lines, verification with holmium oxide filter

Wavelength bunching

1 – 400 nm Programmable in steps of 1 nm

Slit width

1, 2, 4 , 8, 16 nm Programmable slit

Diode width

 < 1 nm

Flow cell

500 nanoliter: 0.5 µl volume, 10 mm cell path length and 50 bar (725 psi) pressure maximum

Maximum pressure

50 bar

Thermostated Column Compartment


Temperature range

10 degrees below ambient to 80 °C

Temperature stability

± 0.15 °C

Column capacity Three

25 cm -

Warm-up/cool-down time

5 minutes from ambient to 40 °C 10 minutes from 40 – 20 °C

Internal volume

3 µl left heat exchanger 6 µl right heat exchanger

Micro Vacuum Degasser


Flow rate

0 – 5 ml/min per channel (5 – 10 ml/min at reduced degassing performance)

Number of channels


Internal volume per channel

Typically 1 ml per channel

Materials in contact with solvent


pH range

1 – 14

Analog output (AUX)

For pressure monitoring, range 0 – 3 V

Evaporation of solvents into the atmosphere

< 200 µg/m3 Acetonitrile and Methanol certification by IAS.

Fraction Collector


Fraction capacities and trays

4 x well-plates full tray (MTP)* (for use with deep well plates, only)

2 × well-plates std. tray (MTP) (for use with deep well plates, only) + 

10 × 2 ml vials* (+ 1 half tray)

 100 x 2 ml in std. tray (+ 1 half tray)

 3 x 40 x 2 ml in half tray*

3 x 15 x 6 ml in half tray*

Full tray with 40 test tubes (30 mm OD, max. height 100 mm, ~45 ml / tube)

Full tray with 60 test tubes (25 mm OD, max. height 100 mm, ~25 ml / tube)

Full tray with 126 test tubes (16 mm OD, max. height 100 mm, ~12 ml / tube)

Full tray with 215 test tubes (12 mm OD, max. height 100 mm, ~7 ml / tube)

test tube / plate sizes

Maximum tube volume ca. 45 ml

Maximum flow rate

10 ml / min (depending on viscosity and generated back pressure, max. 6 bar at the diverter valve)

Delay volumes [μl]

Fraction collector inlet to diverter valve: ~500 (typical, depends on length of the tubing)

Diverter valve: ~15

Diverter valve to needle: ~110

Needle: ~5

maximum capacity 

3 fraction collectors in parallel connected via 12-Position, 13-Port Selector valve (PN G1160A)


  • Agilent 1100 Series LC System
  • Windows 7 Notebook with Chemstation
  • G1315B DAD Detector
  • G1312 Binary Pump
  • G1316 Colcom Thermostatted Column Compartment
  • G1367A WPALS Autosampler
  • G1364 AFC Fraction Collector
  • G1330B ALSTherm Thermostat for Fraction Collector
  • G1379A Vacuum Degasser
  • CAN Cables
  • Power Cord Cord
  • Remote Communication Cable
  • Operator's Guide
  • 30-Day Warranty
  • System has been fully tested by our service team and passed with a clean bill of health

This  Agilent 1100 Series LC System has been fully tested and comes with a clean bill of health. All of our lab equipment comes backed with our standard warranty. Should you have any questions, please don't hesitate to contact us.

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