Agilent 1200 HPLC System with DAD

  • The Agilent 1200 HPLC is available from the lab world group, sellers of top notch used lab equipment
  • The Agilent 1200 HPLC provides you with in depth molecular information in a modular instrument
  • The Agilent 1200 HPLC is simple to operate
  • The Agilent 1200 HPLC brings you a complete analytical solution
  • The Agilent 1200 HPLC has fully integrated hardware and software to boost your productivity
  • The Agilent 1200 HPLC consists of a DAD Detector, binary pump, Thermostated column compartment, autosampler, fraction collector with thermostat and a vacuum degasser
  • The Agilent 1200 HPLC lets you analyze a broad spectrum of compounds
  • The Agilent 1200 HPLC helps shorten analysis times, while providing robust information
  • The Agilent 1200 HPLC lets you do more in a single run with less preparation.
  • The Agilent 1200 HPLC lets you use existing HPLC methods through an orthogonal-spray ion source
  • The Agilent 1200 HPLC uses a high-capacity drying gas system for exceptional sensitivity and reproducibility
  • The Agilent 1200 HPLC eliminates the need to reposition for changes to flow rate or solvent compositions
  • The Agilent 1200 HPLC comes equipped with Chemstation Software, to guide users through a graphical interface
  • The Agilent 1200 HPLC has built in maintenance and validations
  • The Agilent 1200 HPLC allows you to control both data acquisition and set parameters
  • The Agilent 1200 HPLC UV and Mass Spectral data can be processed together
  • The Agilent 1200 HPLC is powerful and simple to maintain
  • The Agilent 1200 HPLC comes with multiple modules that can be swapped out to suit your needs
  • The Agilent 1200 HPLC can be customized to suit your needs
  • The Agilent 1200 HPLC components can be run independently or in concert
  • The Agilent 1200 HPLC is a powerful and flexible tool
  • The Agilent HPLC 1200 is available from The Lab World Group, sellers of top notch used HPLC instruments
  • The Agilent HPLC 1200 provides a modular solution allowing users to customize to meet their Chromatography needs
  • The Agilent HPLC 1200 is modular and compact
  • The Agilent HPLC 1200 is built to boost product development and provide robust analysis
  • The Agilent HPLC 1200 superior resolution, speed and a heightened sensitivity
  • The Agilent HPLC 1200 components can either be used individually through a controlling PC
  • The Agilent HPLC 1200 uses high pressure operation
  • The Agilent HPLC 1200 has a flow rate of up to 10 mL/min for gradient analysis
  • The Agilent HPLC 1200 has programable slit width
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The Agilent 1200 Series HPLC System delivers a highly customizable modular HPLC machine and is the industries most trusted High Pressure Liquid Chromatographs.  The Agilent 1200 can be found in almost every Analytical, R&D, QA, QC, Food & Drug, Biotechnology, Academic, and Pharmaceutical Laboratory thanks to the plug and play design and ease of user.  The 1200 Series HPLC features in-depth diagnostics with Chemstation 32 Bit Software, allowing scientist the ability to do on-site and remote troubleshooting, and allows users to coordinate the modules seamlessly or run modules independently.

The Agilent 1200 is configured for high throughput, with an G1322A Vacuum Degasser, for high performance without the use of helium, a G1311A Quaternary Pump for efficient mixing. The 1200 also comes with a G1316A Thermostated Column Compartment, and a G1315B DAD Diode Array Detector, with 190-950 nm and programable slit width. The G1329A ALS Autosampler has an injection range of between 0.1-100 µl and can replicate injections from 1 – 99 passes from one vial.

Agilent 1200 HPLC System Specifications:

Thermostated Autosampler

Temperature Range

4-40°C in 1° increments

Vacuum Degasser


Flow rate

0-5 ml/min per channel

5-10 ml/min at reduced degassing performance

Number of channels


Internal volume per channel

Typically 1 ml per channel

Materials in contact with solvent


pH range

1 – 14

Analog output (AUX)

For pressure monitoring, range 0 – 3 V

Quart  Pump


Hydraulic system

Dual plunger in series pump with proprietary servo-controlled variable stroke drive, floating plungers and active inlet valve

Setable flow range

0.001 – 10 ml/min, in 0.001 ml/min increments

Flow range

0.2 – 10.0 ml/min

Flow accuracy

± 1% or 10 µl/min whatever is greater

Pressure Operating Range

0 – 40 MPa (0 – 400 bar, 0 – 5880 psi) up to 5 ml/min

Operating range

0 – 20 MPa (0 – 200 bar, 0 – 2950 psi) up to 10 ml/min

Recommended pH range

1.0 – 12.5, solvents with pH < 2.3 should not contain acids which attack stainless steel

ALS Auto Sampler


Injection range

 0.1 – 100 µl in 0.1 µl increments

Replicate injections

1 – 99 from one vial


< 0.25% RSD from 5 – 100 µl, < 1% RSD 1 – 5 µl variable volume

Minimum sample volume

1 µl from 5 µl sample in 100 µl microvial, or 1 µl from 10 µl sample in 300 µl microvial

Carryover Typically

< 0.1%, < 0.05% with external needle cleaning

Sample viscosity range

0.2 – 50 cp

Replicate injections per vial

1 – 99

Sample capacity

100 × 2-ml vials in 1 tray 40 × 2-ml vials in ½ tray 15 × 6-ml vials in ½ tray Agilent vials only)

Injection cycle time

Typically 50 s depending on draw speed and injection volume



Temperature Range

10° below ambient to 80° C

Temperature Stability

± 0.15°C

Temperature Accuracy

± 0.8°C

Column Capacity

Three 30cm columns

Warm Up/Cool Down

5 mins from ambient to 40°C

Dead Volume

3 µl left heat exchanger

6 µl right heat exchanger

DAD Detector


Detector type

1024-element diode array

Light source

Deuterium and tungsten lamp

Short-term noise

< ± 0.8 x 10 -5 AU at 254 nm and at 750 nm


< 0.9 x 10 -3 AU/hr, at 254 nm

Wavelength range

190-950 nm

Wavelength accuracy

± 1 nm, self-calibration with deuterium lines, verification with holmium oxide filter

Slit width

Programmable: 1, 2, 4, 8, 16 nm

Diode width

< 1 nm

Wavelength bunching

Programmable, 2 - 400 nm, in steps of 1 nm

Flow cells



13-µL volume, 10 mm cell path length, 120 bar (1760 psi) pressure maximum


5-µL volume, 6 mm cell path length, 120 bar (1760 psi) pressure maximum


2-µL volume, 3 mm cell path length, 400 bar (5880 psi) pressure maximum


500-nanoliter volume 10-mm cell path length, 50 bar (735 psi) pressure maximum


80 nanoliter volume., 6-mm cell path length, 50 bar (735 psi) pressure maximum


3-mm cell path length, 120 bar (1760 psi) pressure maximum

High pressure

1.7-µL volume, 6-mm cell path length, 400 bar (5880 psi) pressure maximum

Time programmable

Wavelength, polarity, peak width, lamp bandwidth, autobalance, WL range, threshold, spectra storage mode

Spectral tools

Data analysis software for spectra evaluation, including spectral libraries and peak purity functions

Analog output

Recorder/integrator 100 mV or 1 V, 2 outputs

  • Agilent 1200 HPLC System
  • G1322A Vacuum Degasser
  • G1311A Quat Pump
  • G1315B DAD Diode Array Detector
  • G1316A TCC Thermostatted Column Compartment
  • G1329A ALS Autosampler
  • G1330B ALS Thermostat
  • Windows 7 Notebook with Chemstation
  • Full System Diagnostics Report with Clean Bill of Health
  • CAN Communication Cables
  • 120V Power Cords
  • Operator's Guide
  • 30-Day Warranty

This Agilent 1200 HPLC System has been fully inspected and comes with validations for all of the components. All of our lab equipment comes backed with our standard warranty. Should you have any questions, please don't hestiate to contact us.

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