Agilent 1200 Series HPLC System with DAD

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The Agilent 1200 Series HPLC System is a must have for an analytical lab. The modular and compact design of the HPLC System allows users to configure to their needs while taking up a minimal amount of bench space. This HPLC system features a powerful combination of speed, resolution, and sensitivity with a high throughput and improved quality of results. The 1200 series HPLC system uses a plug and play modular system, so modules automatically hand shake with each other, or can be used separately depending on your needs. The Agilent 1200 Series HPLC instrument uses a dual lamp design and temperature control through diode array detectors to bring you outstanding sensitivity. This is also partnered with a high pressure binary pump for flexible solvent delivery, and a degasser for stable flow rate. The thermostat auto sampler of this high-performance liquid chromatography machine has a temperature range of between 4-40°C, as well as a high-performance auto sampler with an injection range of between 0.1-100 µl.

The Agilent 1200 Series HPLC System consists of a G1379B Vacuum Degasser, a G1312A Binary Pump, a G1329A Autosampler, a G1330B ALS ThermostatG1316A TCC Thermostatted Column Compartment and a G1315D DAD Detector. The Diode Array Detector allows for flexible slit settings for a range of 1 to 16 nm, letting you customize the desired sensitivity, linearity and spectral resolution. This HPLC System, built for purification and analysis, also comes with a controlling PC installed with Chemstation software to help manage and interpret large amounts of data collected from the system. The Chemstaion software is windows based, and user friendly and gives you level 5 control of your instrument with built in diagnostics and early warnings for maintenance. Thanks to the scalability and easy maintenance of this LC System, this work horse will rise to meet your needs for years to come.

Agilent 1200 Series HPLC System Specifications:

Thermostated Autosampler


Temperature Range

4-40°C in 1° increments

DAD Detector


Detection type

1024-element photodiode array

Light source

Deuterium and tungsten lamps

Wavelength range

190 – 950 nm

Short term noise

< ± 0.8 x 10 -5 AU at 254 nm and at 750 nm


< 0.9 x 10 -3 AU/hr, at 254 nm

Linear absorbance range

> 2 AU (upper limit)

Wavelength accuracy

± 1 nm Self-calibration with deuterium lines, verification with holmium oxide filter

Wavelength bunching

1 – 400 nm Programmable in steps of 1 nm

Slit width

1, 2, 4 , 8, 16 nm Programmable slit

Diode width

 < 1 nm

Flow cell Standard

13-µL volume, 10 mm cell path length, 120 bar (1760 psi) pressure maximum

Vacuum Degasser


Flow rate

0-5 ml/min per channel

5-10 ml/min at reduced degassing performance

Number of channels


Internal volume per channel

Typically 1 ml per channel

Materials in contact with solvent


pH range

1 – 14

Analog output (AUX)

For pressure monitoring, range 0 – 3 V

Binary  Pump


Hydraulic system

Dual plunger in series pump with proprietary servo-controlled variable stroke drive, floating plungers and active inlet valve

Setable flow range

0.001 – 5 ml/min, in 0.001 ml/min increments

Flow range

0.1 – 5.0 ml/min

Flow accuracy

± 1% or 10 µl/min whatever is greater

Pressure Operating Range

Setpoints 0.001 – 5 ml/min, in 0.001 ml/min increments

Pressure pulsation

< 2 % amplitude (typically < 1 %), at 1 ml/min isopropanol, at all pressures > 1 MPa

Recommended pH range


1.0 – 12.5, solvents with pH < 2.3 should not contain acids which attack stainless steel

ALS Auto Sampler


Injection range

 0.1 – 100 µl in 0.1 µl increments

Replicate injections

1 – 99 from one vial


< 0.25% RSD from 5 – 100 µl, < 1% RSD 1 – 5 µl variable volume

Minimum sample volume

1 µl from 5 µl sample in 100 µl microvial, or 1 µl from 10 µl sample in 300 µl microvial

Carryover Typically

< 0.1%, < 0.05% with external needle cleaning

Sample viscosity range

0.2 – 50 cp

Replicate injections per vial

1 – 99

Sample capacity

100 × 2-ml vials in 1 tray 40 × 2-ml vials in ½ tray 15 × 6-ml vials in ½ tray Agilent vials only)

Injection cycle time

Typically 50 s depending on draw speed and injection volume


Temperature Range

10° below ambient to 80° C

Temperature Stability

± 0.15°C

Temperature Accuracy

± 0.8°C

Column Capacity

Three 30cm columns

Warm Up/Cool Down

5 mins from ambient to 40°C

Dead Volume

3 µl left heat exchanger

6 µl right heat exchanger





100-120/220-240 V, 50/60 Hz



  • Agilent 1200 HPLC System with
  • G1379B Vacuum Degasser
  • G1312A Binary Pump
  • G1315D DAD Detector
  • G1316A TCC Thermostatted Column Compartment
  • G1329A Autosampler
  • G1330B ALS Thermostat
  • Solvent Tray
  • Windows 7 Notebook with Chemstation
  • System Validation Report with Clean Bill of Health
  • Operator's Guide
  • CAN Communication Cables
  • 120V Power Cords
  • 30-Day Warranty

This Agilent 1200 Series HPLC System has been thoroughly inspected, with tests run on each individual component and a pressure test on the system as a whole.  All of our lab equipment comes backed with our standard warranty. Should you have any questions, please do not hesitate to contact us.

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