• BioTek Synergy 2 Plate Reader available from The Lab World Group, sellers of quality used lab equipment.
  • BiOTek Synergy 2 plate reader ideal for laboratories working with small bench space, PMT, fluorescence reading, tungsten halogen lamp, Xenon Flash lamp
  • BioTek Synergy 2 plate reader works in luminescence mode, dual optics, monochromator, constant light,
  • BioTek Synergy 2 reader runs in 200 to 700 nm works with BioStack Robot
  • BioTek Synergy 2 comes equipped with dual optics
  • The BioTek Synergy 2 in a multi detection microplate readers used for a wide range of studies
  • The BioTek Synergy 2 comes with a Windows 7 Notebook running Gen5 software
  • The BioTek Synergy 2 is a multi-detection microplate reader designed to read in fluorescence, absorbance and luminescence
  • The BioTek Synergy 2 in absorbance mode, the microplate reader switches to different Xenon Flash Lamp which allow the detection of UV and visible light
  • the Synergy 2 is also compatible with a wide range of automated systems included the BioTek BioStack system
  • The Bio-Tek Synergy 2 Multi-Detection Microplate Reader is high-performance, modular and built for drug discovery
  • The biotek Synergy 2 works with microplates from 6-1526
  • the Synergy 2 works with volumes from 5-1000 uL
  • the Synergy 2 uses absorbance fluorescence and luminescence to get results
  • the Synergy 2 is ready to ship

Biotek Synergy 2 Multi-Mode Plate Reader (SLFPTA)


Microplate Readers



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The BioTek Synergy 2 is a high-performance multi-mode plate reader built for drug discovery (R&D) and the biotech life science research arena. This single-channel microplate reader uses a combination of monochromator, filters and dichroic mirrors for the superior performance in research and screening assays. Detection modes included with this Synergy 2 plate reader is an SLFPTA model that includes are Fluorescence Intensity, Top and Bottom, Polarization, and Time-Resolved Fluorescence, Luminescence, and UV-Vis Absorbance.

The BioTek Synergy 2 Multi-Mode Microplate Reader uses a long-life tungsten halogen lamp for determining Fluorescence detections, with both top and bottom reading capability. The luminescence of this Biotek plate reader is measured with a low noise PMT, and absorbance measuring is made with a xenon flash lamp that allows for both UV and Visible light. In addition, this Biotek microplate reader has 4 zone temperature control, with a range of between 4-50°C, as well as three speeds of shaking.

The BioTek Synergy 2 Plate Reader is controlled by a computer using BioTek’s Gen 5 Software which is compatible with AlphaScreen assays and certified for HTRF, DLR, and LantaScreen. The Synergy 2 is robot accessible and compatible with BioTek’s BioStack Microplate Stacker. Gen5 supports OLE automation to facilitate the Synergy 2’s integration into an automated system. Contact us today with any questions about our Biotek Synergy microplate readers.

Biotek Synergy 2 Model SLFA Specifications: 

Detection Method

Absorbance, Fluorescence (top and bottom), Luminescence

Microplate types

6- to 1536-well plates (luminescence 1-384) PCR plates Compatible with Take3 Multi-Volume Plate with 2 µL microspot



Volume Range:

5-1000 µL with a 5-20 µL tip-prime



Measurement Range

0.000 to 4.000 OD

Scanning Speed

96: 11 seconds, 384: 22 seconds, 1536: 42 seconds

Wavelength Range

200 to 999 nm 1 nm increment


± 2 nm

Wavelength Selection



2.4 nm


Photodiodes (2) Measurements are reference channel-corrected for light source fluctuation


50 amol/well flash ATP in a 96-well plate

500 amol/well flash ATP in a 96-well plate



Excitation Filter:

485/20 nm

Emission Filter:

528/20 nm

Samples per well



96-well read: 99 sec

384-well read: 339 sec

1536-well read: 1256 sec

Time-Resolved Fluorescence


96/384-well plates:

250 fM Europium (plate and well modes), 20 reads per location, 5 mm Z-axis offset, 350 ms delay before read

Integration Interval:

20 to 16000 µs


0 to 16000 µs


1-µs steps

Fluorescence Polarization


96/384-well plates

5 mP at 1 nM Sodium Fluorescein, 60 reads per location, 5 mm Z-axis offset 350 ms delay before read



Absorbance: Xenon flash light source, 10W maximum average

power, lamp life 1 billion flashes (not user-changeable)

Fluorescence: Tungsten quartz halogen, 20W power, lamp life

1000 hours (user-changeable)

TRF (and optionally for FI and FP): Xenon flash light source, 60W maximum average power, lamp life 1 billion flashes (not user-changeable)


Temperature control range from 4º over ambient to 50ºC. Temperature variation ± 0.50°C across the plate @ 37°C (250 µl per well with the plate sealed).

Plate Shaking:

Low, Medium, High and Variable shaking speeds. Shake time is programmable by the user.


18 x 17 x 15”


24-volt external power supply compatible with

100-240 V~; +10% @50-60 Hz


35 lbs

  • Biotek Synergy 2 Multi-Mode Plate Reader | CAT# SLFPTA | MSRP: 48,332.00
  • Windows 10 Laptop Running Gen5 
  • Absorbance Validation Report
  • Fluorescence Validation Report
  • Communication Cable
  • Biotek Synergy User Manual
  • 120V Power Cord (12A)
  • 30-Day Warranty
  • Please Note: Microplate Reader has been fully tested, calibrated, and validated by our service team with a clean bill of health.

This BioTek Synergy 2 Microplate Reader has been fully inspected and comes with current validations and systems check. All of our lab equipment comes backed with our standard warranty. Should you have any questions about this or any of our other Microplate Readers, please don't hesitate to contact us.

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