BioTek Synergy 4 Multi-Mode Microplate Reader

Microplate Readers
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The BioTek Synergy 4 Multi Detection Microplate Reader provides a user-friendly solution for a wide range of microplate read options. This plate reader combines monochromator based capabilities with filter based detection for the best in flexibility. This Microplate reader is the complete synergy 4 model with Temperature control, plate shaking, monochromator based fluorescence intensity (top), Luminescence, Filter Based Fluorescence Intensity (top and bottom) Fluorescence Polarization, Time resolved fluorescence, UV-Vis absorbance and a dual reagent dispenser, this instrument has you covered.

The BioTek Synergy 4 Multi Detection Microplate Reader accommodates all standard microplates with 128 x 86 mm geometry, 96-well half-area plates, and the Take3 Multi-Volume Plate, though maximum plate height varies by application, and luminescence measurements are not supported for 1536 formats. The dispensing volume of this plate readers is between 5 and 1000 µl in 1 µl increments, and features temperature control at 4°C above ambient to 50°C ± 0.5°C at 37°C.

BioTek Synergy 4 System Specifications:

Detection Mode

Absorbance (UV/Vis), Luminescence, Fluorescence

Temperature Control

4°C above ambient to 50°C ± 0.5°C at 37°C


Yes, Built-in

Microplate Formats

Monochromator system: 1- to 384-well plates

Filter system: 1- to 1536-well plates

(luminescence 1 - 384)


2 syringe pumps


Dispenser Volume

5–1000 μL with a 5–20 μL tip prime

Dead Volume

1.1ml, 100 µl with backflush

 Absorbance Specifications:

Light Source

Xenon flash light source, 10W maximum average power, lamp

life 1 billion flashes (not user-changeable)

Wavelength Selection


Wavelength Range

200 - 999nm, 1nm increments


2.4 nm

Dynamic Range

0 - 4.0 OD


0.001 OD

Monochromator Wavelength Accuracy


Monochromator Wavelength Repeatability


Fluorescence Specifications:


Xenon flash light source, 60W maximum average

power, lamp life 1 billion flashes (not user-changeable)

Tungsten quartz halogen, 20W power, lamp life

1000 hours (user-changeable)


Monochromators: fluorescein 5 pM typical (0.5 fmol/well 384-well plate) Filters/mirrors: fluorescein 1 pM typical (0.1 fmol/well 384-well plate)

Wavelength Range

EX 250–700 nm, EM 300–800 nm

Wavelength Selection

Double grating monochromators (Top) and, deep blocking bandpass filters / dichroic mirrors (Top/Bottom)

Detection System

Multi Label

Reading Speed

96-well 42 seconds, 384-well 147 seconds, 1536-well 543 seconds

Luminescence Specifications:


10 amol ATP typical (flash)

Wavelength Range

300 - 700 nm

Dynamic Range

> 6 decades

Biotek Synergy 4 System Breakdown:


Temperature control range from 4º over ambient to 50ºC

(readers with serial number 217299 and higher and basecode

version 1.14 and later can incubate to 65ºC). Temperature

variation ± 0.50°C across the plate @ 37°C (250 μL per well with

the plate sealed).

Plate Shaking

Low, Medium, High, and Variable shaking speeds. Shake time is

programmable by the user.


100 - 240 VAC 50/60Hz


20.9" D x 15" W x 17.5" H


45 lbs

  • BioTek Synergy 4 Multi Detection Microplate Reader
  • Biotek Synergy 4 Model S4MLFPTAD
  • Temperature Control Up to 65°C
  • Absorbance Monochromator
  • Fluorescence Monochromator (Top)
  • Luminescence
  • Filter Base Fluorescence (Top & Bottom)
  • Fluorescence Polarization
  • Time Resolved Fluorescence 
  • Abs Validation Report with clean bill of health
  • FL Validation Report with clean bill of health
  • Operator's Guide
  • Communication Cable
  • 115V Power Adapter
  • 30Day Warranty

This BioTek Synergy 4 Multi Detection Microplate Reader has been fully inspected and comes with current validation reports and a clean bill of health. All of our lab equipment comes backed with our standard warranty. Should you have any questions, please don't hesitate to contact us.

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