• Biotek Synergy H4 Multi-Mode Microplate Reader available from The Lab World Group
  • The Biotek Synergy H4 is a reliable multi label microplate reader that uses a monochromator platform to analyze data
  • The Biotek Synergy H4 comes with Gen5 software used for data collection and analysis
  • The Biotek Synergy H4 is a flexible low cost multi mode microplate reader
  • The BioTek Synergy H4 multi-mode plate reader offers unmatched performance
  • The Biotek Synergy H4 is a high throughput multiple detection microplate reader built on a high performance hyrbrid monochormator platform
  • The Biotek Synergy H4 has the ability to read in Absorbance and Fluorescence Intensity
  • The Biotek Synergy H4 uses a quadruple grating optics allowing for excitation and emission wavelength to be stepped in 1 nm increments
  • The Biotek Synergy H4 microplate reader is ideally suited for a wide variety of research assays you may find in a life sciences research laboratory
  • The Biotek Synergy H4 microplate reader is a modular design which can be fully armed by adding modules for Time-Resolved fluorescence (TRF), TR-FRET, fluorescence intensity, FRET, Fluorescence Polarization, AlphaScreen/AlphaLISA and Absorbance
  • The Biotek Synergy H4 microplate reader is configured with monochromator absorbance, top and bottom monochromator-based fluorescence, top filter-based fluorescence
  • The Biotek Synergy H4 microplate reader was built with drug discovery in mind
  • The Biotek Synergy H4 microplate reader allows for low limits detection in even high density microplates while bringing uncompromised detection throughout a wide range of applications

Biotek Synergy H4 Hybrid Microplate Reader H4MFPTA

Category:

Microplate Readers

Manufacturer:

Bio-tek

SKU:
BIOTEKSYNERGYH4HBRIDPLATEREADER-9378
Ships Within:
3 Business Days
Qty Available:
1
Price:
$11,499

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  • EQUIPMENT DESCRIPTION
  • SPECIFICATIONS
  • INCLUDED
  • NOTES

The Biotek Synergy H4 Multimode Microplate Reader is a Hybrid system.  The Biotek Plate Reader incorporates monochromator-based optics with a sensitive filter-based optics, delivering a high-performance platform for fluorescence-based applications. This modular microplate reader an ideal choice for labs dealing in research and development, drug discovery and other fields where performance and adaptability are crucial. This multi-label high-performance hybrid monochromator-based optics give users the ultimate in flexibility of wavelength choice from low UV to near IR.  Unlike the Synergy 2, the Biotek Synergy H4 is a modular design to adapt to your needs. The Filter dichroic based optics deliver unmatched lighting and purification for quick wavelength switching along with reading speeds.  The Biotek Synergy H4 Hybrid Microplate Reader is configured with monochromator absorbance, top and bottom monochromator-based fluorescence, top filter-based fluorescence, TRF, and FP. The microplate reader runs off a Xenon Flash Lamp as well as a tungsten lamp and has a wavelength accuracy of ± 2nm.


The Biotek Synergy H4 was built with drug discovery in mind.  This high-performance Biotek microplate reader allows for low limits detection in even high-density microplates while bringing uncompromised detection throughout a wide range of applications.  Some of these assays include Kinase, Ion Channel, Enzyme and GPCR.  The Biotek Synergy is both HTRF and LantahScreen Plus Certified and a great tool for those looking to take their drug discovery to the next level.  The Synergy Plate Reader is compatible with the Biotek Take3 nanoplates, allowing for smaller micro samples to be used, features temperature control up to 65°C, and plate shaking.  The Synergy H4 uses quadruple grating optics allowing for excitation and emission wavelength to be stepped in 1 nm increments. The filter-based optics of this plate reader use dichroic mirrors that are faster and more sensitive than the monochromator optics.

Biotek Synergy H4M System Specifications:

Absorbance

 

Wavelength Range

230 to 999 nm 1 nm increment

Wavelength Selection

Monochromator

Light Source

Xenon Flash

Bandwidth

2 nm (230-285 nm), 4 nm (>285 nm)

Dynamic Range

0 – 4.0 OD

Resolution

0.0001 OD

Pathlength Correction

Yes

Monochromator wavelength accuracy

± 2 nm

Monochromator wavelength repeatability

± 0.2 nm

OD accuracy

<1% at 2.0 OD typical <3% at 3.0 OD typical

OD linearity

<1% from 0 to 3.0 OD typical

Stray Light

0.03% at 230 nm typical

Scanning Speed

96: 11 seconds, 384: 22 seconds

Fluorescence Intensity

 

Monochromators:

Fluorescein 2.5 pM typical (0.25 fmol/well 384-well plate) – Top
Fluorescein 5 pM typical (0.5 fmol/well 384-well plate) – Bottom

Filters/mirrors:

Fluorescein 1 pM typical (0.1 fmol/well 384-well plate)

Wavelength Selection

Double Grating monochromators (Top/Bottom) and,

Deep blocking fliters/dichroic mirrors (top)

Wavelength Range

Monochromators: 250-850 nm

Filters: 200-700 nm (850 nm option)

Dynamic Range

Monochromators: 5 decades

filters/mirrors: > 6 decades

Bandpass

Monochromators: variable 9, 13.5, 17, 20nm

Filters: filter-dependant, from 5nm to >100nm

Detection System

Red shifted PMT for monochromator system

Low Noise PMT for filter-based system

Lighting

Tungsten halogen and Xenon Flash

Reading Speed

96: 11 seconds

384: 22 seconds

1536: 42 seconds

Fluorescence Polarization

 

Light Source

Tungsten Halogen

Sensitivity

3 mP at nM fluorescein typical

Wavelength Selection

Deep blocking filters / dichroic mirrors (top)

Wavelength Range

400-700 nm (320-850 nm option)

Time-Resolved Fluorescence

 

Light Source

High Energy Xenon Flash Lamp

Sensitivity

Europium 60 fM typical with Filters (6 amol/well in 384-well plate)

Wavelength Selection

Double Grating monochromators (Top/Bottom) and,

Deep blocking fliters/dichroic mirrors (top)

Wavelength Range

Filters: 200-700 nm (850 nm option)

Monochromators: 250-850 nm

General

 

Detection Method

UV-Visible Absorbance

Top and Bottom Fluorescence – Monochromator

Top Fluorescence – Filter

Fluorescence Polarization

Detection Mode

Multi-Label

Read Method

End-point, kinetic, spectral scanning, well-area scanning

Sample Formats

Monochromator system 1- to 384-well plates

Filter system 1- to 1536-well plates PCR plates

Compatible with Take3 Multi-Volume Plate with 2 µL microspots

Shaking

Yes

Control

Gen5 Software (Windows 7 Notebook)

Dimensions (W x D x H)       

18 x 21 x 15”

Power

100 - 240V AC 50/60HZ

Weight

70 lbs.

 

 

  • Biotek Synergy H4 Hybrid Microplate Reader | MSRP: $52,290.00
  • Computer Running Gen5 Software
  • Absorbance Validation / Calibration Report (230-999nm Wavelength Range / Monochromator)
  • Fluorescence Validation Report (250-850nm Wavelength Range / Monochromator)
  • Excitation Filters Included: 360/40, 485/20, 540/25
  • Emission Filters Included: 460/40, 528/20, 620/40
  • Mirrors Included:
  • 50% Excitation 200-850nm & Emission 200-850nm
  • Dichroic Top 400nm Excitation 320-390nm & Emission 410-800nm
  • Dichroic Top 510nm Excitation 440-505nm & Emission 515-640nm
  • Probe Size Included:
  • Absorbance Probe 1.4mm
  • Top Monochromator Probe 2.0mm
  • Bottom Monochromator Probe 2.0mm
  • Top Filter Probe 3.5mm
  • Communication Cable
  • Biotek Synergy User Manual
  • 30-Day Warranty

This Biotek Synergy H4 Hybrid Microplate Reader has been fully tested and validated by our service team.  The Biotek Synergy is complete with a computer and comes with a calibration report showcasing a clean bill of health.  Microplate Reader ships out within three business days by way of freight services.  

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