Molecular Devices SpectraMax M5 Microplate Reader

Category:
Microplate Readers
Manufacturer:
Molecular Devices
Listing #:
MOLECULARDEVICESSPECTRAMAXM5EREADER-5701
Ships Within:
3 Business Days

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  • EQUIPMENT DESCRIPTION
  • SPECIFICATIONS
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The Molecular Devices Spectramax M5 Multimode Microplate Reader provides you with 5 modes of detection each as accurate as would be a dedicated single mode reader. This microplate reader uses the powerful PathCheck Sensory technology to measure the depth of samples without being temperature dependent. When used with Soft Max Pro, the Path Check Sensor of this microplate reader will normalize the well absorbance, letting users calculate directly without absorptive compounds. This microplate reader uses a dedicated photomultiplier tube to reduce stray light and boost the best signal to noise. For luminescence reading by using Instead of lenses this multimode microplate reader uses elliptical mirrors that maximize transmission, while minimizing wavelength distortion. This multi-detection plate reader also features customizable spreadsheets and powerful graphing tools.

 

The Molecular Devices Spectramax M5 Multimode Microplate Reader features two monochromators allowing users to define their optimal excitation and emissions without the need to change filters. This multimode microplate reader is capable of Absorbance, Fluorescence Intensity, luminescence, Time resolved fluorescence, fluorescence polarization, and covers formats from cuvettes to 6-, 12-, 24-, 48-, 96-, and 384-well microplates. This microplate reader also incorporates a triple-mode cuvette port, accurate temperature control, and microplate shaking. The Molecular Devices Spectramax M5 Multimode Microplate Reader features easy to set up and control with 140 preprogramed protocols getting you up and running fast. This multimode microplate reader is robot compatible as well to help boost productivity and automation. 

Molecular Devices SpectraMax M5 Microplate Reader Specifications:

Robotic-compatible

Yes

Plate formats

6, 12, 24, 48, 96, 384 wells

Light source

Xenon Flash Lamp (1 joule/flash)

Detectors

2 photomultiplier tubes (PMT)

Shaker time

0 to 999 seconds

Temp. control

2°C above ambient to 60°C

Temp. uniformity

< 1°C at 37°C set point

Temp. accuracy

±1°C at 37°C set point

Readings

Endpoint, Kinetic Spectral Scanning

Standard Read Times (minutes:seconds)* 96 wells 384 wells

 

Absorbance 0:18 0:49

Fluorescence Intensity 0:17 0:48

Luminescence 2:00 7:00

Absorbance Photometric Performance

 

Wavelength range

200–1000 nm

Wavelength selection

Monochromator, tunable in 1.0 nm increments

Wavelength bandwidth

≤ 4.0 nm

Wavelength accuracy

±2.0 nm

Wavelength repeatability

±0.2 nm

Photometric range

0–4.0 OD

Photometric resolution

0.001 OD

Photometric accuracy

(microplate) < ±0.006 OD ±1.0%, 0–2 OD

 (cuvette) < ±0.005 OD ±1.0%, 0–2 OD

 

Photometric precision

< ±0.003 OD ±1.0%, 0–2 OD

Stray light

< 0.05% @ 230 nm

Fluorescence Intensity Performance

Top or bottom

Wavelength range

250–850 nm

Wavelength selection

Monochromators, tunable in 1.0 nm increments

Bandwidth (EX, EM)

9 nm, 15 nm

Optimized sensitivity

≤ 1 pM fluorescein in 96 wells,

≤ 1.5pM in 384 wells

Guaranteed sensitivity

< 5 pM fluorescein in 96 wells or cuvette,

 < 20 pM in 384 wells

Luminescence Performance

Top or Bottom

Wavelength selection

Choice of simultaneous detection of all wavelengths or selection via monochromator, tunable in 1.0 nm increments

Wavelength range

250–850 nm

Optimized sensitivity

≤ 43pM ATP in 96 wells

Guaranteed sensitivity 

≤ 75pM ATP in 96 wells

Dynamic range

> 6 decades

Cross-talk

< 0.3% in white 96- and 384-well Microplates

Dimensions

23 x 16 x 9 inches

Weight

36 lbs

Power

< 420 watts, 100–240 VAC, 3.5 A, 50/60Hz

Time Resolved Fluorecesnce Performance

Top or Bottom of a Microplate

Wavelength selection Monochromators,

tunable in 1.0 nm increments

Bandwidth (EX, EM)

9 nm, 15 nm

Precision data collection

1–100 flashes, delay of 0–600 µsec. before read, integration time selectable between 50–1500 µsec.

Optimized sensitivity

≤ 10 fM europium in 96

Guaranteed sensitivity

≤ 100 fM europium in 96 or 384 wells

SpectraMax M5 Reader only

Certified to Cisbio Bioassays HTRF technology performance specifications

Fluorescence Polarization Performance

 

Wavelength range

300–750 nm

Wavelength selection

Monochromators, tunable in 1.0 nm increments

Bandwidth (EX, EM)

9 nm, 15 nm

Optimized Precision

≤ 3.5 mP standard deviation at 1 nM fluorescein in 96 wells

Guaranteed Precision

< 5 mP standard deviation at 1 nM fluorescein in 96 wells

  • Molecular Devices Spectramax M5 Microplate Reader
  • Windows 7 Notebook with Original SoftMax Pro 7.0 
  • Absorbance Validation Report with Clean Bill of Health
  • Fluorescence Validation Report with Clean Bill of Health
  • Communication Cable
  • 120V Power Cord
  • Operator's Guide
  • Fluorescence Top Reading Purple Plate Adapter
  • 30-Day Warranty

This Molecular Devices Spectramax M5 Multimode Microplate Reader has been fully inspected and comes with current validation reports and clean bills of health. All of our lab equipment comes backed with our standard warranty. Should you have any questions, please don't hesitate to contact us.

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