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Molecular Devices SpectraMax M2 Multimode Plate Reader
- EQUIPMENT DESCRIPTION
The Molecular Devices SpectraMax M2 is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 6-384 microplate reading capability. This easy to use multimode plate reader is designed to handle the workflow of multiple research projects. Detection modes include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer, or spectrophotometer. The SpectraMax M2 can do Endpoint, Kinetic based assays, Spectrum, and area-well scanning read types and is also equipped to do PathCheck. Path check allows homogeneous and heterogeneous microplate assays to be performed in this flexible system. In addition, the system can also act as an ELISA Reader and comes with built-in assays that are easily accessible through the SoftMax Pro software.
The SpectraMax M2 reduces the cost for expensive filters by using a dual monochromator platform. This eliminates the need for any add-on excitation or emission filters. The filter wheel is already built inside so there’s no need to swap or change them out. The two scanning monochromators to define the optimal excitation and emission settings, allowing a quick click-through process to change out any methods, or fluorophores, making your process more efficient. The built in PathCheck Sensor then measures the optical pathlength of samples within a microplate and automatically normalizes the well absorbance to a cuvette equivalent of 1cm. This is like using 96 or 384 cuvettes simultaneously. This technology gets rid of standard curves and detects compounds with a known extinction coefficient, allowing absorbance to calculate the concentration within the sample.
The Multimode reader comes with a built-in cuvette port allows easy conversion and optimization of very-low-throughput assays into medium-high-throughput microplate assays. The Molecular Devices SpectraMax M2 allows faster, more precise results and reagent savings. In addition, combined absorbance and fluorescence intensity assays can be run by issuing a single read command. This plate reader is a great choice for applications such as DNA / RNA / Protein Quantitation and Purity, PicoGreen / NanoOrange / Bradford, Cytotoxicity Assays, ELISA Plate, and Enzyme Kinetics, Drug Dissolution Profiles, and much more.
SpectraMax M2 Specifications:
Absorbance photometric performance
Monochromator, tunable in 1 nm increments
≤ 4.0 nm
Photometric accuracy (microplate)
< ±0.006 OD ±1.0%, 0–2 OD
Photometric accuracy (cuvette)
< ±0.005 OD ±1.0%, 0–2 OD
< ±0.003 OD ±1.0%, 0–2 OD
< 0.001 OD
< 0.05% @ 230 nm
Fluorescence photometric performance
1 nm increment selection
EX Wavelength range
EM Wavelength range
Wavelength bandwidth (EX, EM)
Top-read optimized sensitivity
5 pM (fluorescein) in 96 wells
Top-read guaranteed* sensitivity
15 pM (fluorescein) in 96 wells
Time-Resolved Fluorescence (secondary mode)
50–1450 µsec., 200 µsec. increments
7 pM Eu-chelate
Luminescence (secondary mode)
Wavelength range (M2)
50 fM alkaline phosphatase
General photometric performance
6, 12, 24, 48, 96, 384 wells
Xenon flash lamp (1 joule/flash)
Photomultiplier tube (PMT)
Read time** 96 wells: 384 wells:
Abs 18 sec., FI 15 sec. Abs 49 sec., FI 45 sec.
0 to 999 seconds
Ambient +4°C to 45°C
< 1°C at 37°C set point
±1°C at 37°C set point
< 125 watts
100–240 Vac, 3 A, 50/60 Hz
21” x 16” x 9”
- SpectraMax M2
- Laptop with SoftMax Pro 6
- Absorbance Validation Report with Clean Bill of Health
- Fluorescence Validation Report with Clean Bill of Health
- Communication Cable
- 120V Power Cord
- 30-Day Warranty
This system has been fully tested by our service team and comes with a clean bill of health including current validations, and managing software. All of our lab equipment comes backed with our standard warranty. Should you have any questions, please don't hesitate to contact us.