ABI StepOne Plus Real Time PCR Machine

PCR Thermal Cyclers
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The ABI StepOnePlus is an advanced Real Time PCR used for fast quantitative measurements.  The Applied Biosystems StepOne Plus PCR Machine has the ability to measure gene amplifications as they’re occurring in real-time, cycle by cycle, increasing precision measurements during the vital stage of the PCR process.  The instrument comes with a built-in touch screen which allows you to manually set up and programs runs.  In addition, the ABI StepOne Plus comes with a Windows 7 focused application software for deep analysis which can decipher experimental results in real time.   The software allows you to quickly setup real-time PCR processes, thermal cycling, and fluorescence detection and reaction setup.  The Applied Biosystems StepOnePlus from Life Technologies now owned by Thermo Scientific delivers a twofold discrimination with 99.7% accuracy in addition to nine logs of dynamic range. The system can decipher 5,000 and 10,000 template copies.

This real-time PCR is capable of detecting 10 copies of RNase P in a 30µl reaction.  System includes SMART technology capable of ordering reagents.  The StepOnePlus Real-Time PCR Machine system comes equipped with a 96 well block with flexibility to run four color dyes at once.  Moreover, the Applied Biosystems StepOnePlus is equipped with fluorophore detection which includes TaqMan, FAM, VIC, TAMRA, MGB Probe Based Assays, SYBR Green, and much more.  The PCR is ideal for MicroRNA Expression, Gene Detection, SNP Genotyping, Gene Expression Profiling, Viral Load Analysis, Mutation Scanning, Translocation Analysis, and Methylation.  In conjunction with the software the system is capable of measuring melting curves with High Resolution Melting, better known as HRM.  Contact us to learn more about our ABI StepOne Plus Real Time PCR system.   

StepOne Plus qPCR Specifications:



Excitation Source

Blue LED


4 filters factory calibrated  

Dynamic Range

9 logs of linear dynamic range

Multiplex Quantitation

SYBR Green dye, TaqMan


96-well plates, 0.1ml tubes

Fast Block


Temperature Uniformity

± 0.5c within 30 seconds of clock start

Demonstrated Sensitivity

9 logs of linear dynamic range


23.6" x  51.2" x 42.7" 

Power Requirements

115V 50/60Hz


53 lbs

  • Applied Biosystems StepOnePlus PCR Machine
  • Windows 7 Notebook with StepOne Software
  • Communication Cable
  • Power Cord
  • Operator's Guide
  • Diagnostics Report
  • 30-Day Warranty

These ABI StepOne Plus Real Time PCR Machine have been fully inspected and come with current validation reports. All of our lab equipment comes backed with our standard warranty. Should you have any questions, please don't hesitate to contact us.

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