BioRad CFX384 Touch Real Time PCR

PCR Thermal Cyclers
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The Biorad CFX384 Real-Time delivers accurate quantification for up to five targets within large volume reactions.  The qPCR can also perform target discrimination as well.  The Bio-Rad CFX delivers the latest Real-Time PCR technology while providing a platform which can run as a stand alone system, or software driven if so desired.  In order to perform reproducible results, each sample well of the Real-Time PCR must maintain uniform temperature throughout each incubation step.   The BioRad CFX384 does so by utilizing it's six independent thermal electric modules which are controlled either through the CFX Manager software, or by the built-in microprocessor controls provided with this qPCR. These controls not only manage heating, but also the cooling element of this system, even during ramping of the Thermal Cycler. The BioRad CFX joins the company of the Bio-Rad CFX Touch and Deepwell Touch as one of the highest sought after Real-Time PCR systems on today's market.  It's optical reaction module converts the BioRad C1000 LCD  thermal cycler chassis into the powerful and precise system which dramatically shortens thermal cycle run times into minutes in stead of hours.  The CFX Manager provided with the BioRad CFX384 is extremely easy to use and comes equipped with built-in protocols required for Real-Time PCR reactions.  

Reagents can be purchased at Bio-Rad along with Applied Biosystems as some of their PCR reagents work with this system. The CFX Manger software from BioRad allows you to set the optimal temperature of your experiments by monitoring and tracking results in real-time using the built-in gradient thermal cycle feature.  With the Bio Rad CFX you can program up to 24°C throughout the reaction block during any step in your assay.  This provides unsurpassed temperature uniformity along with reproducibility in every gradient zone. In addition, the Bio-Rad CFX Real-Time PCR allows for quick setup with factory calibrated dyes.  Users can easily monitor real-time data on the LCD Screen, or within the CFX Manager software.  Having this feature allows the user to make quick adjustments to your protocols which in the end will result in higher productivity.  Moreover, the data collected from the qPCR system can easily be modified and quickly exported to a number of different file extensions.  All in all this system will allow you toquickly set up runs and monitor amplification traces in real-time on the integrated C1000 base with the Bio-Rad CFX.   The system is extremely easyt to use, power and will make for high quality results.  Contact us today to learn more about the BioRad CFX384 Real-Time PCR.

BioRad CFX384 Real-Time PCR Specifications:

Max. ramp rate

2.5°C / per second

Average ramp rate

2°C / per second

Heating & Cooling Method



Heats up to 105°C

Temperature Range


Temperature Accuracy

±0.2 of programmed target at 90°C

Temperature Uniformity 

±0.4 well-to-well within 10 sec of arrival at 90°C

BioRad CFX qPCR Gradient Operational Range

30 - 100°C

Gradient Programmable Span

1 - 24°C

Optical Detection Excitation

5 Filtered LEDs

Optical Detection

5 Filtered Photodiodes

Range of Excitation / Emission Wavelengths

450 - 690 nm


Detects 1 copy of target sequence

in human genomic DNA

Dynamic Range

10 orders of magnitude

Scan Time All Channels

< 20 seconds

FAM/SYBR Green only on this Real-Time PCR

8 seconds

Sample Capacity 

96 wells

Sample Size

1-30µl (5 - 20 recommended)

Communication Interface

USB 2.0


33 (w) x 46 (d) x 36(h) cm


13" x 18" x 14" inches


21kg / 47lb

  • BioRad CFX384 Real-Time PCR System
  • BioRad C1000 Base
  • Windows 7 Laptop Running CFX Manager Software
  • Power Cord
  • Operator's Guide
  • USB Cable
  • Warranty

This listing is for a slightly used Biorad CFX Real Time PCR.  The system has been fully tested and validated by our service department.  This qPCR has passed all system diagnostic and spectral test, instrument is in excellent working condition.

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