ABI StepOne Real Time PCR System

Description

ABI StepOne Real Time PCR System

The Applied Biosystems StepOne Real-Time PCR was specifically designed to streamline the process of qPCR.  This quantitative real-time PCR comes with an easy-to-use touchscreen graphical user interface (GUI) allowing the user to quickly start an experimental run without using the computer. This advanced Real-Time PCR works well with TaqMan Gene Expression and Power SYBR green assays bringing the next level of PCR to first-time users.  The ABI StepOne delivers quantitative analysis during amplification, cycle by cycle, in real-time, during a key phase of PCR.  This is ideal for researchers looking to tweak their methods on the fly. This qPCR is cost-efficient to use with a 3 color/ 48 well format for precise, quantitative real-time PCR results that performs standard and fast PCR reactions in less than 40 minutes. This PCR system also features automated SNP genotype-calling, auto-baseline and auto-threshold for simplified data analysis, and a long-life LED-based optical system.

In addition, the homepage of the ABI StepOne qPCR allows the user to quickly navigate and choose different Real-Time PCR methods which include thermal cycling, fluorescent detection, and reaction setup.  Moreover, this real-time PCR comes with a built-in design wizard to help the researcher build their custom methods.  Just click on the Applied Biosystems Design Wizard, answer a few questions, and let StepOne do the rest.  The Wizard guides you through template types such as ramp speeds, nucleic acid, quantitation method, detection chemistry, and more.  The Design Wizard will also ask you to choose the plate layout (48 well, or PCR strips) along with the reaction mix and standard dilution calculations.

This ABI StepOne qPCR can also be controlled through PC Stepone software allowing users to view multiple plots for simultaneous assessing of data from four perspectives. The ABI Software is extremely easy to use and works with the ABI 7500 FAST and Applied Biosystems StepOnePlus Real-Time PCR as well.  This PCR machine also includes a USB Flash Stick port to allow the researchers to take their results with them.  You can save your data on the computer and create customer-looking graphs and reports which are easily exported to Excel or Microsoft PowerPoint.

The ABI StepOne can process fluorophore detection chemistries including FAM and VIC dye-labeled TaqMan probe-based assays, and SYBR Green I dye chemistry. The TaqMan probe-based tests deliver outstanding specificity and sensitivity needed for accurate Real-Time PCR analysis. The SYBR Green dye kits are an economical alternative for target identification, initial screening assays, or methods that require only a few reactions. StepOne qPCR applications include a standard curve for absolute PCR quantitation and relative quantitation.  The application includes Relative standard curve and comparative, as well as genotyping, multiplexed assays, and melt curve analysis.