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BioRad CFX384 Real Time PCR System

$6,495

SKU: 24923-25334 Category:
Clear

Included with ID 24923-25334
BioRad CFX384 Real Time PCR | CAT# 1855484 | MSRP: $49,738.05
CFX384 Optics Module | CAT# 1845384
C1000 Touch Thermal Cycler Base | CAT# 1841100
Windows 10 Notebook Running CFX Maestro Software v2.3
System Test Validation
Communication Cable
User Manual
120V Power Cord (7A)
30-Day Warranty

2 in stock

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Description

BioRad CFX384 Real-Time PCR

The Biorad CFX384 Real-Time accurately quantifies up to four targets within large-volume reactions.  The qPCR can also perform target discrimination as well.  The BioRad CFX Real-Time PCR features five filtered LEDs and 5 filtered photodiodes to collect data from all the wells and detect up to 4 targets per well.  To perform reproducible results, each sample well of the Real-Time PCR must maintain a uniform temperature throughout each incubation step. The Bio-Rad CFX384 utilizes its six independent thermal electric modules, controlled either through the CFX Manager software or by the built-in microprocessor controls provided with this qPCR.

These controls manage the heating and cooling elements of this system, even during the Thermal Cycler’s ramping. The BioRad CFX optical reaction module converts the BioRad C1000 Touch LCD thermal cycler chassis into a powerful and precise system that dramatically shortens thermal cycle run times into minutes instead of hours. The fast scan option reads single-channel fluorescence in all 384 wells in 8 seconds. The CFX Manager provided with the BioRad CFX384 is extremely easy to use and has built-in protocols required for Real-Time PCR reactions.

The CFX Manager software from BioRad allows you to set the optimal temperature of your experiments by monitoring and tracking results in real time using the built-in gradient thermal cycle feature.  With the Bio-Rad CFX, you can program up to 24°C throughout the reaction block during any step in your assay.  This provides unsurpassed temperature uniformity along with reproducibility in every gradient zone. In addition, the Bio-Rad CFX Real-Time PCR allows for quick setup with factory-calibrated dyes.  Users can easily monitor real-time data on the LCD Screen or within the CFX Manager software.

 This feature allows the user to make quick adjustments to your protocols, resulting in higher productivity.  Moreover, the data collected from the qPCR system can easily be modified and quickly exported to several different file extensions.  This system will allow you to quickly set up runs and monitor amplification traces in real-time on the integrated C1000 base with the Bio-Rad CFX.   The system is straightforward to use and powerful, producing high-quality results. Should you have any questions about this Bio-Rad CFX384 real-time PCR system, please don’t hesitate to contact us.

Specifications

BioRad CFX384 Real-Time PCR Specifications:

Max. ramp rate 2.5°C / per second
Average ramp rate 2°C / per second
Heating & Cooling Method Peltier
Lid Heats up to 105°C
Temperature Range 0-100°C
Temperature Accuracy ±0.2 of programmed target at 90°C
Temperature Uniformity ±0.4 well-to-well within 10 sec of arrival at 90°C
BioRad CFX qPCR Gradient Operational Range 30 – 100°C
Gradient Programmable Span 1 – 24°C
Optical Detection Excitation 5 Filtered LEDs
Optical Detection 5 Filtered Photodiodes
Range of Excitation / Emission Wavelengths 450 – 690 nm
Sensitivity Detects 1 copy of target sequence

in human genomic DNA

Dynamic Range 10 orders of magnitude
Scan Time All Channels < 20 seconds
FAM/SYBR Green only on this Real-Time PCR 8 seconds
Sample Capacity 96 wells
Sample Size 1-30µl (5 – 20 recommended)
Communication Interface USB 2.0
Dimensions 14x19x15″
Power  100-240 VAC, 50/60 Hz, 850W
Weight 45lbs

Included

Included with BioRad CFX384 Real-Time PCR

Please see individual SKU ID’s for a complete list of included items.

Notes

Technical Notes

This Biorad CFX Real Time PCR has been fully tested and validated by our service department. This qPCR has passed all system diagnostic and spectral tests, the instrument is in excellent working condition. All of our lab equipment comes backed with our standard warranty. Should you have any questions, please don’t hesitate to contact us.

Video

BioRad CFX384 Real-Time PCR Video

ID 24923-25334

BioRad CFX384 Real Time PCR System Video ID 24770

ID 23100

Biorad CFX384 Real Time PCR ID 17102