- Description
- Specifications
- Included
- Notes
- Video
Description
Description
BioTek Synergy Neo2 Multi-Mode Plate Reader
The BioTek Synergy Neo2 Hybrid Multimode Reader gives your lab a sleek, flexible instrument with automated capabilities. This microplate reader features two independent optical paths, variable-bandwidth quad monochromators, filter-based optics, laser TRF, and multiple PMTs for fast results. The monochromator system can be set between 230-999 nm in 1 nm increments, and the swappable filter cubes allow you to customize the system to meet your lab’s requirements. The high-quality filter cubes of this plate reader use a barcode system to register the configuration automatically, and you’re off and running. These cubes’ fiberless design also means powerful sample excitation and high sensitivity.
The hybrid technology of this BioTek Synergy Neo2 gives readers independent optical paths, with continuously variable bandwidth quad monochromators, laser-based excitation, and filter-based optics for sensitivity. This system is outfitted with Monochromator ABS, FLU mono and filter-based with top and bottom reading, fluorescence polarization, TRF, LUM and Alpha laser installed.
To increase the efficiency of your workflow, the NEO2 can be partnered with a NEO Biostack Robot Handler (available from the manufacturer). This plate stacker can transfer a plate in just 6 seconds and can be controlled through the Gen5 software. This robust software helps guide users through setup and compliance with the 21 CFR part and GxP requirements, allows for multiple user profiles and permission levels, and collects and analyzes data.
Specifications
Specifications
BioTek Synergy Neo2 Multi Mode Plate Reader Specifications:
| Detection mode | Quad monochromators: FL, Lum., UV-Vis Abs., TRF (secondary) Filters: FL, TRF, FP, Lum., Alpha, TR-FRET, BRET |
| Read mode | End point, kinetic, spectral scanning, well area scanning |
| Microplate types | 1- to 1536-well plates |
| Other labware | Compatible with Take3™ Micro-Volume Plates with 2 µL |
| Temperature control | To 65 ºC with Condensation Control |
| Variation | ±0.2 ºC at 37 ºC |
| Shaking | Linear, orbital, double orbital |
| Software | Gen5 Microplate Reader and Imager Software included
Gen5 Secure Software option for 21 CFR Part 11 compliance features |
| Automation | BioStack and 3rd party automation compatible BioSpa 8 Automated Incubator compatible |
| Barcode reader | Multi-directional, 1D and 2D camera-based |
| Read height | Auto Z, 0.1 mm steps, top/bottom (Filters), top (Mono) |
| Kinetic speed | 96-well: 6 seconds; 384-well: 11 seconds; 1536-well: 25 seconds |
| With Neo Stacker minimum processing time per plate | 96-well: 20 seconds
384-well: 25 seconds 1536-well: 39 seconds |
| Absorbance | |
| Light source | Xenon flash lamp |
| Wavelength selection | Monochromator |
| Wavelength range | 230 – 999 nm, 1 nm increment |
| Bandwidth | 2 nm (230 – 285 nm), 4 nm (>285 nm) |
| Dynamic range | 0 – 4.0 OD |
| Resolution | 0.0001 OD |
| Fluorescence Intensity | |
| Sensitivity: Filter Cubes | Fluorescein 0.2 pM (384-well low volume plate) – Top
Fluorescein 1 pM (1536-well plate) – Top Fluorescein 1 pM (384-well plate) – Bottom |
| Quadruple Monochromator | Fluorescein 2 pM (384-well low volume plate) – Top
Fluorescein 2.5 pM (384-well plate) – Bottom |
| Light source | High energy xenon flash lamp |
| Read height | Auto Z, 0.1 mm steps, top/bottom (Filters), top (Mono) |
| Wavelength selection | Double grating monochromators (top/bottom)
Filters (top/bottom) |
| Wavelength range | Monochromators: 250 – 850 nm |
| Filters Cubes | 360/460 nm
485/528 nm |
| Monochromator | |
| Bandwidth | Variable, from 3 nm to 50 nm in 1 nm increment excitation/emission |
| Detection system | Single PMT or dual PMTs (top filter system)
Low Noise PMT bottom filter system Red shifted PMT top/bottom monochromator system |
| Luminescence | |
| Sensitivity | 5 amol ATP (flash) (384-well low volume plate) |
| Wavelength range | 300 – 700 nm |
| Dynamic range | >6 decades |
| Fluorescence Polarization | |
| Sensitivity | 1 mP standard deviation at 1 nM fluorescein (384-well low volume plate) 1.5 mP standard deviation at 1 nM fluorescein (1536-well plate) |
| Light source | Xenon flash lamp |
| Wavelength selection | Filter cubes (top/bottom) |
| Wavelength range | 280 – 850 nm |
| Detection system | Single PMT or dual PMTs |
| Time-Resolved Fluorescence | |
| Light source | Xenon flash lamp |
| Sensitivity | Europium 40 fM (384-well low volume plate)
Europium 70 fM (1536-well plate) |
| Wavelength selection | Filter Cubes (top/bottom) Double grating monochromator (top/bottom) |
| Wavelength range | Monochromators: 250 – 850 nm |
| Filters | 200 – 850 nm |
| Detection system | Single PMT or dual PMTs |
| Alpha | |
| Sensitivity | 100 amol LCK peptide (384-well low volume plate) |
| Light source | 100 mW 680 nm laser |
| Wavelength selection | Filter cubes |
| Read speed | 96-well: 30 seconds
384-well: 1 minute 50 seconds 1536-well: 7 minutes 20 seconds |
| Dimension | 20x15x16- Plate Reader
18x7x27- Biostacker |
| Weight | 80lbs |
| Power | 120V Power Cords (12A), 250 Watts max |
Included
Included
Included with this Microplate Reader
Biotek Synergy Neo2 Multi-Mode Microplate Reader | CAT# NEO2MALPHAB | MSRP: $96.498.13
Windows 10 Laptop Running Gen5 Ver. 3.08 Software
Absorbance Validation Report
Fluorescence Validation Report
User Manual
Communication Cables
120V Power Cords (12A)
30-Day Warranty
Notes
Notes
Technical Notes
This Biotek Plate reader has been thoroughly inspected and is operating without issue.
Please Note: Biotek Neo2 system has been fully tested and passed absorbance, fluorescence and luminescence validations. The plate reader has a lot of options; if the buyer wants to add more filters later on, they can purchase them directly from the manufacturer.
All of our lab equipment comes backed by our standard warranty. Should you have any questions, please don’t hesitate to contact us.
Video
Video
BioTek Synergy Neo2 Multi Mode Plate Reader Video
Biotek Synergy Neo2 MultiMode Plate Reader Video 25460
