Lonza 4D Nucleofector Electroporation

$9,495$11,495

SKU: 23955 Category:
Clear

Included with ID 23955
Lonza 4D Nucleofector Electroporation System | MSRP: $33,239.41
Touch Screen Controller
4D-Nucleofector Core Unit
4D-Nucleofector LV Unit
4D-Nucleofector X Unit
Communication Cable
User Manual
120V Power Cord
30-Day Warranty

$11,495

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Description

Lonza 4D Nucleofector Electroporation

The Lonza 4D Nucleofector Electroporation is a modular transfection system. Nucleofection helps overcome almost all transfection challenges through tailored kits and optimizing electrical parameters. This 4d nucleofector is comprised of a Core unit with touch screen operation, an x unit that holds 2 100ul nucleocuvette vessels, and an LV Unit designed to expand the system to large-scale transfection. The LV unit is closed and scalable, working with larger cell numbers from 1×107 to 1×109 cells.

The 4d nucleofector comes loaded with cell-type-specific optimized protocols and access to more than 680 entries in the online Cell Transfection. Nucleofection also allows for the transfection of adherent cells and cells in suspension. The Lonza 4d nucleofector is also a very flexible system that can apply the same parameters to almost any substrate, making it ideal for over-expression studies, the generation of stable clones, and more.

The Lonza 4D Nucleofector Electroporation is operated through a user-friendly touch screen or controlled through a PC with software. The flexibility of this system allows users to work with neural cells in adherence at a later developmental stage, non-viral transfection of primary neural cells with up to 70% efficiency, and it preserves functionality. Users can also work with primary and cell lines related to metabolic diseases with up to 90% efficiency while maintaining viability.

Specifications

Lonza 4D Nucleofector Electroporation Specifications:

Capacity 16-32 reactions (using 16-well Nucleocuvette  Strips)

12-24 reactions (using 100 µL Nucleocuvette  Vessels)

2 Reactions (using 1ml LV Nucleocuvette Cartridge)

1-5 reactions (using LV Nucleocuvette Cartridge, up to 20ml reaction volume)

Throughput (samples per run) Low to medium (1 – 16) Throughput (samples per run) Low to medium (1 – 16)
Reaction volume 20 µl + 100 µl Reaction volume 20 µl + 100 µl
/ voltage limitation 1500 V
current limitation 100 A
Electrode material Conductive polymer Electrode material Conductive polymer
Low cell numbers (20 µl) 5 × 104 to 1 × 105
High cell numbers (100 µl) 2 × 105 to 2 × 106
DNA vector amount/sample 0.2 – 1 μg (20 µl) 1 – 5 μg (100 µl) DNA vector amount/sample 0.2 – 1 μg (20 µl) 1 – 5 μg (100 µl)
LV Unit
Throughput (samples per run) 1-5
Reaction volume 20ml
Cells/Sample 1×107 to 1×109 cells
Dimensions 11x10x17” all together
Weight 33lbs
Power 100-240v, 50-60Hz,

Included

Included with this Nucleofector System

Please see individual SKU ID’s for a complete list of included items.

Notes

Technical Notes

This nucleofector system has been fully inspected and is operating without issue. All of our lab equipment comes backed by our standard warranty. Should you have any questions, please don’t hesitate to contact us.

Video

Lonza 4D Nucleofector Video

ID 23955

Lonza 4D Nucleofector Electroporation ID 23955

ID 24654

Lonza 4D Nucleofector Electroporation ID 24654