Molecular Devices Spectramax M5e Multi Mode Microplate Reader

    
Molecular Devices Spectramax M5e Multi Mode Microplate Reader
Category:
Microplate Readers
Manufacturer:
Molecular Devices
Listing #:
MOLECULARDEVICESSPECTRAMAXM5EREADER-3246
Ships Within:
3 Business Days

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  • EQUIPMENT DESCRIPTION
  • SPECIFICATIONS
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  • NOTES

The Molecular Devices Spectramax M5e Multimode Microplate Reader offers you accuracy of a dedicated single mode reader across 5 modes of detection including being certified for Cisbio Bioassays HTRF technology. This microplate reader features two monochromators allowing users to define their optimal excitation and emissions without the need to change filters. This multimode microplate reader is capable of Absorbance, Fluorescence Intensity, luminescence, Time resolved fluorescence, fluorescence polarization, HTRF and covers formats from cuvettes to 6-, 12-, 24-, 48-, 96-, and 384-well microplates. This microplate reader also incorporates a triple-mode cuvette port, accurate temperature control, and microplate shaking.


The Molecular Devices Spectramax M5e Multimode Microplate Reader can measure the depth of samples without being temperature dependent thanks to the powerful PathCheck Sensory technology. When used in combination with the comprehensive data management software Soft Max Pro, the Path Check Sensor of this microplate reader will normalize the well absorbance, letting users calculate directly without absorptive compounds. This microplate reader boosts the best signal to noise for luminescence reading by using a dedicated photomultiplier tube to reduce stray light. Instead of lenses this multimode microplate reader uses elliptical mirrors that maximize transmission, while minimizing wavelength distortion.


The Molecular Devices Spectramax M5e Multimode Microplate Reader comes with dedicated data acquisition and analysis software, making it easy to set up and control with 140 preprogramed protocols getting you up and running fast. These ready-to-run protocols include HTRF assays from Cisbio Bioassays and LanthaScreen TR-FRET assays. This multimode microplate reader is robot compatible as well to help boost productivity and automation. This multi-detection plate reader also features customizable spreadsheets and powerful graphing tools.

Molecular Devices Spectramax M5e Multimode Microplate Reader Specifications:

Robotic-compatible

Yes

Plate formats

6, 12, 24, 48, 96, 384 wells

Light source

Xenon Flash Lamp (1 joule/flash)

Detectors

2 photomultiplier tubes (PMT)

Shaker time

0 to 999 seconds

Temp. control

2°C above ambient to 60°C

Temp. uniformity

< 1°C at 37°C set point

Temp. accuracy

±1°C at 37°C set point

Readings

Endpoint, Kinetic Spectral Scanning

Standard Read Times (minutes:seconds)* 96 wells 384 wells

 

Absorbance 0:18 0:49

Fluorescence Intensity 0:17 0:48

Luminescence 2:00 7:00

Absorbance Photometric Performance

 

Wavelength range

200–1000 nm

Wavelength selection

Monochromator, tunable in 1.0 nm increments

Wavelength bandwidth

≤ 4.0 nm

Wavelength accuracy

±2.0 nm

Wavelength repeatability

±0.2 nm

Photometric range

0–4.0 OD

Photometric resolution

0.001 OD

Photometric accuracy

(microplate) < ±0.006 OD ±1.0%, 0–2 OD

 (cuvette) < ±0.005 OD ±1.0%, 0–2 OD

 

Photometric precision

< ±0.003 OD ±1.0%, 0–2 OD

Stray light

< 0.05% @ 230 nm

Fluorescence Intensity Performance

Top or bottom

Wavelength range

250–850 nm

Wavelength selection

Monochromators, tunable in 1.0 nm increments

Bandwidth (EX, EM)

9 nm, 15 nm

Optimized sensitivity

≤ 1 pM fluorescein in 96 wells,

≤ 1.5pM in 384 wells

Guaranteed sensitivity

< 5 pM fluorescein in 96 wells or cuvette,

 < 20 pM in 384 wells

Luminescence Performance

Top or Bottom

Wavelength selection

Choice of simultaneous detection of all wavelengths or selection via monochromator, tunable in 1.0 nm increments

Wavelength range

250–850 nm

Optimized sensitivity

≤ 43pM ATP in 96 wells

Guaranteed sensitivity 

≤ 75pM ATP in 96 wells

Dynamic range

> 6 decades

Cross-talk

< 0.3% in white 96- and 384-well Microplates

Dimensions

23 x 16 x 9 inches

Weight

36 lbs

Power

< 420 watts, 100–240 VAC, 3.5 A, 50/60Hz

Time Resolved Fluorecesnce Performance

Top or Bottom of a Microplate

Wavelength selection Monochromators,

tunable in 1.0 nm increments

Bandwidth (EX, EM)

9 nm, 15 nm

Precision data collection

1–100 flashes, delay of 0–600 µsec. before read, integration time selectable between 50–1500 µsec.

Optimized sensitivity

≤ 10 fM europium in 96

Guaranteed sensitivity

≤ 100 fM europium in 96 or 384 wells

SpectraMax M5e Reader only

Certified to Cisbio Bioassays HTRF technology performance specifications

Fluorescence Polarization Performance

 

Wavelength range

300–750 nm

Wavelength selection

Monochromators, tunable in 1.0 nm increments

Bandwidth (EX, EM)

9 nm, 15 nm

Optimized Precision

≤ 3.5 mP standard deviation at 1 nM fluorescein in 96 wells

Guaranteed Precision

< 5 mP standard deviation at 1 nM fluorescein in 96 wells

Dimensions

23x16x9 "

Weight

36 lbs
Power 100-240V, 50/60 Hz, 3.5A

  • Molecular Devices Spectramax M5e Multimode Microplate Reader
  • Windows 7 Notebook with Software Max Pro 6.2.2 
  • Absorbance Validation Report
  • Fluorescence Validation Report
  • Operator's Guide
  • Communication Cable
  • Power Cables
  • 30-Day Warranty

This Molecular Devices Spectramax M5e Multimode Microplate Reader has been fully inspected by our team comes with current validation reports. All of our lab equipment comes backed with our standard warranty. Should you have any questions, please don't hesitate to contact us.

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