• The Thermo Scientific Varioskan Flash is available from The Lab World Group
  • The Thermo Scientific Varioskan Flash is a robust Multimode Microplate Reader capable of running a wide range of measurements from low UV to near IR
  • The Thermo Scientific Varioskan Flash users can optimize their assays
  • The Thermo Scientific Varioskan Flash can read in absorbance, luminescence, and fluorescence and includes detection for photometric, luminometric, time resolved fluorescence, and fluorescence intensity.
  • The Thermo Scientific Varioskan Flash offers unlimited wavelength selection with up to three onboard dispensers used for optimal performance, meeting the demands of today’s laboratory needs
  • The Thermo Scientific Varioskan Flash  offers unlimited wavelength selection with up to three onboard dispensers used for optimal performance, meeting the demands of today’s laboratory needs.
  • The Thermo Scientific Varioskan Flash has the ability to run Kinetic, ADMETox, Cell Signaling, enzyme, and ion-channel assays making it extremely desirable by researchers worldwide
  • The Thermo Scientific Varioskan Flash end users can set up assays to run in low UV to near IR with a few clicks of a mouse.
  • The Thermo Scientific Varioskan Flash is ideally suited for direct measurement of proteins, RNA, and DNA due large in part to the Photometric Pathlength correction
  • The Thermo Scientific Varioskan Flash works well with label-free assays used for protein structure changes by using Fluorometric UV measurements of fluorescent amino acids.
  • The Thermo Scientific Varioskan Flash has a top and bottom reading feature
  • The Thermo Scientific Varioskan Flash delivers high-performance cellular assays without interference from biological background noise
  • The Thermo Scientific Varioskan Flash has Time-Resolved Fluorescence Resonance Energy Transfer, also known as TR-FRET, can be quickly optimized by using the TRF spectral scanning feature
  • The Thermo Scientific Varioskan Flash allows the users to easily tweak their measurements for the best possible results
  • The Thermo Scientific Varioskan Flash is the luminometric monochromator mode delivering the most optimized wavelength measurement in luminometric Multilable assays

Thermo Varioskan Flash Multi Detection Microplate Reader

Category:

Microplate Readers

Manufacturer:

Thermo Scientific

SKU:
THERMOVARISKANFLASHPLATEREADER-10836
Ships Within:
3 Business Days

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The Thermo Scientific Varioskan Flash is a robust Multimode Microplate Reader capable of running a wide range of measurements from low UV to near IR, so users can optimize their assays. This spectral scanning multimode microplate reader can read in absorbance, luminescence, and fluorescence and includes detection for photometric, luminometric, time resolved fluorescence, and fluorescence intensity.  This Thermo Varioskan Flash microplate reader offers unlimited wavelength selection with up to three onboard dispensers used for optimal performance, meeting the demands of today’s laboratory needs. The plate reader is also has a DLR Luminescence Feature already installed and ready to go. 


The easy to use multi-detection microplate reader has the ability to run Kinetic, ADMETox, Cell Signaling, enzyme, and ion-channel assays making it extremely desirable by researchers worldwide.  Given the Thermo Scientific Varioskan Flash has full wavelength scanning, end users can set up assays to run in low UV to near IR with a few clicks of a mouse.   In addition, this spectral scanning multimode microplate reader is ideally suited for direct measurement of proteins, RNA, and DNA due large in part to the Photometric Pathlength correction. The system works well with label-free assays used for protein structure changes by using Fluorometric UV measurements of fluorescent amino acids.  The Fluorescent cell-based applications deliver optimal performance with the Thermo Scientific Varioskan Flash top and bottom reading feature along with the built-in high precision incubator.   The flexibility multi-mode microplate reader accommodates a broad range of microplates and is compatible with the new Thermo Scientific µDrop nanoplates. 


With the built-in Time-Resolved Fluorescence technology, the Varioskan Flash delivers high-performance cellular assays without interference from biological background noise.  The Time-Resolved Fluorescence Resonance Energy Transfer, also known as TR-FRET, can be quickly optimized by using the TRF spectral scanning feature.  TRF delay time optimization can be achieved by using automatic τ value calculation allowing the users to easily tweak their measurements for the best possible results. Another nice feature on the Thermo Scientific Varioskan Flash is the luminometric monochromator mode delivering the most optimized wavelength measurement in luminometric Multilable assays. Runs can be performed with greater sensitivity with the normal, or filter mode of the dedicated Varioskan LumiSens optics.


The Thermo Varioskan includes the powerful Skant software, enhanced for easy assay setup, flexible data handling, and convenient report formatting.  The SkanIt software comes with a built-in assay wizard that assists the user in a step-by-step process permitting for seamless workflow needed in today’s lab environment.  The Thermo SkantIt software is 21 CFR Part 11 compliant and feature audit trail recording required by the FDA. Contact us for more information on the Thermo Scientific Varioskan Flash Multi-Mode Microplate Reader. 

Thermo Scientific Varioskan Flash System Specifications:

Fluorescence Intensity/TRF                                                                                                                                                                                                                                                                                                                                                                                      
Plate types6 – 1536-well plates
Wavelength selectionDouble excitation and double emission monochromators
Excitation wavelength range200 – 1000 nm
Emission wavelength range270 – 840 nm
Excitation/emission bandwidth5 nm and 12 nm/12 nm
Light sourceXenon flash lamp
Sensitivity/dynamic rangeFluorescence intensity, top reading: < 0.4 fmol fluorescein/well, > 6 decades, 384-well plate Fluorescence intensity, bottom reading: < 4 fmol fluorescein/well, > 5.5 decades, 384-well plate Time-resolved fluorescence, top reading: < 120 amol Europium/well, > 6 decades, 384-well plate
Luminometry
Plate types6 – 1536-well plates, spectral scanning 6 – 384-well plates
Wavelength selectionAll wavelengths, filters and double monochromators
Wavelength range360 – 670 nm, spectral scanning 270 – 840 nm
Sensitivity/dynamic range< 7 amol ATP/well, > 7 decades, flash ATP reaction, 384-well plate
Photometry
Plate types6 – 1536-well plates, spectral scanning 6 – 384-well plates
Wavelength selectionAll wavelengths, filters and double monochromators
Wavelength range360 – 670 nm, spectral scanning 270 – 840 nm
Sensitivity/dynamic range< 7 amol ATP/well, > 7 decades, flash ATP reaction, 384-well plate
Photometry
Plate types6 – 384-well plates
Wavelength selectionDouble monochromators
Wavelength range200 – 1000 nm
Bandwidth5 nm
Light sourceXenon flash lamp
Linear measurement range0 – 4 Abs (96-well plate) at 450 nm, ± 2% 0 – 3 Abs (384-well plate) at 450 nm, ± 2%
Accuracy± 2% or 0.003 Abs, whichever is greater, at 200 – 399 nm (0 – 2 Abs) ± 1% or 0.003 Abs, whichever is greater, at 400 – 1000 nm (0 – 3 Abs)
PrecisionSD < 0.001 Abs or CV < 0.5%, whichever is greater, at 450 nm (0 – 3 Abs)
Dispenserup to 3, automatic dispensing position control
Plate types6 – 384-well plates
Syringe size1 ml (standard), 5 ml (on request)
Dispensing volume1 – 10 000 µl, with 1 µl increments (1 ml syringe) Automatic safety control based on maximum well volume
Accuracy< 0.2 μl or 2%, whichever is greater, 5 – 10 000 μl (1 ml syringe, 0.40 mm tip)
Precision5 – 19 μl < 5%, 20 – 10 000 μl < 2% (1 ml syringe, 0.40 mm tip)
Dispensing speed30 s, 96-well plate 80 s, 384-well plate (5 µl/well, 1 ml syringe, 0.40 mm tip)
IncubatorFrom ambient + 4°C to 45°C at ambient 25°C
Shaker                                                                                                                                                                          Orbital with adjustable speed and diameter
General Features
Measurement modesEndpoint, kinetic, spectral scanning with all detection technologies
Measurement speed96-well plate in 15 s, 384-well plate in 45 s, and 1536-well plate in 135 s (minimum kinetic interval time from A1 back to A1)
Spectral scanning speed< 2 s/well, 400 – 500 nm, 1 flash, 2 nm steps
Power100-240V 50/60Hz, 200VA
Dimensions (W x D x H)20x22x20"
Weight65lbs

  • Thermo Varioskan Flash Multi-Detection Microplate Reader
  • Windows 10 Laptop Running SkanIt Software
  • Absorbance Validation Report
  • Fluorescence Validation Report
  • Absorbance
  • Fluorescence (Top & Bottom Read) 
  • DLR Luminescence Feature Installed
  • Communication Cable
  • 120V Power Cord (12A)
  • 30-Day Warranty

This listing is for a slightly used Thermo Scientific Varioskan Flash plate reader that's been fully inspected and comes with current validations. All of our lab equipment comes backed by our standard warranty. Should you have any questions, please don't hesitate to contact us.

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