Molecular Devices SpectraMax M2E Microplate Reader

Microplate Readers
Molecular Devices
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The Molecular Devices SpectraMax M2E provides powerful multi detection reading for microplates and cuvettes in a flexible, dual monochromator, easy to use instrument. The M2e plate reader is a great choice for applications in DNA, RNA and Protien quantitation and purity checks, enzyme kinetics, drug dissolutions profiles, cytotoxicity assays and many more. This multi-mode plate reader features dual cuvette ports and is compatible with microplates from 6 to 384 wells. The built-in cuvette ports make for easy conversion and optimization of low-throughput assays into medium-high-throughput microplate assays. The Spectramx M2e has a broad wave length range of 250-850 nm, and detects in absorbance, fluorescence intensity, top and bottom fluorescence readings as primary modes, with time-resolved fluorescence, and luminescence as secondary modes.

The SpectraMax M2E Microplate Reader is low cost to operate, thanks to the dual monochromator platform, that eliminates the need for expensive add on excitation or emission filters. The already incorporated filter wheel, and scanning monochromators define the optimal excitations and emission settings, with the PathCheck sensor to measure the optical pathlength of samples in a microplate. The PathCheck sensor allows for homogeneous and heterogeneous microplate assays by getting rid of standard curves and detecting compounds with known extinction coefficient, allowing absorbance to calculate the concentration within the sample. This flexible reader system automatically normalizes the well absorbance to a cuvette equivalent of 1cm. The Spectramax M2e system can also act as an ELISA Reader and comes with built-in assay which are easily accessible through the SoftMax Pro software. The SpectraMax M2E Microplate Reader can do Endpoint, Kinetic, Spectrum and area-well scanning read types. This microplate reader can also operate as well as a dedicated spectrofluorometer, or spectrophotometer. In addition, the system can also act as an ELISA Reader and comes with built-in assay which are easily accessible through the SoftMax Pro software.

SpectraMax M2E Plate Reader Specifications:

Absorbance photometric performance


Wavelength selection

Monochromator, tunable in 1 nm increments

Wavelength range

200–1000 nm

Wavelength bandwidth

≤ 4.0 nm

Wavelength accuracy

±2.0 nm

Wavelength repeatability

±0.2 nm

Photometric range

0–4.0 OD

Photometric resolution

0.001 OD

Photometric accuracy (microplate)

< ±0.006 OD ±1.0%, 0–2 OD

Photometric accuracy (cuvette)

< ±0.005 OD ±1.0%, 0–2 OD

Photometric precision

< ±0.003 OD ±1.0%, 0–2 OD

Baseline flatness

< 0.001 OD

Stray light

< 0.05% @ 230 nm

Fluorescence photometric performance


Dual monochromators

1 nm increment selection

EX Wavelength range

250–850 nm

EM Wavelength range

250–850 nm

Wavelength bandwidth (EX, EM)

9 nm

Top-read optimized sensitivity

5 pM (fluorescein) in 96 wells

Top-read guaranteed sensitivity

15 pM (fluorescein) in 96 wells

Bottom-read optimized sensitivity

10 pM (fluorescein) in 96 wells

Bottom-read guaranteed sensitivity

25 pM (fluorescein) in 96 wells

Time-Resolved Fluorescence (secondary mode)


Wavelength range

250–850 nm

Data collection

50–1450 µsec., 200 µsec. increments

Guaranteed sensitivity

7 pM Eu-chelate

Luminescence (secondary mode)


Wavelength range (M2E)

250–850 nm

Guaranteed sensitivity

50 fM alkaline phosphatase

General photometric performance


Plate formats

6, 12, 24, 48, 96, 384 wells

Light source

Xenon flash lamp (1 joule/flash)


Photomultiplier tube (PMT)

Read time** 96 wells: 384 wells:

Abs 18 sec., FI 15 sec. Abs 49 sec., FI 45 sec.

Shaker time

0 to 999 seconds

Temperature control

Ambient +4°C to 45°C

Temperature uniformity

< 1°C at 37°C set point

Temperature accuracy

±1°C at 37°C set point

General specifications


Power Consumption 

< 125 watts


100–240 Vac, 3 A, 50/60 Hz


21” x 16” x 9”



  • Molecular Devices SpectraMax M5 Multi-Detection Plate Reader
  • Windows 7 Notebook with SoftMax Pro 6.2.2
  • Absorbance Validation Report
  • Fluorescence Validation Report
  • Operator's Guide
  • Communication Cable
  • 115V Power Cord
  • 30-Day Warranty

This Molecular Devices SpectraMax M2E Microplate Reader has been fully inspected and comes with current validations reports. All of our lab equipment comes backed with our standard warranty. Should you have any questions, please don't hesitate to contact us.


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