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Molecular Devices SpectraMax M2E Microplate Reader
Microplate Readers
Molecular Devices
- EQUIPMENT DESCRIPTION
- SPECIFICATIONS
- INCLUDED
- NOTES
The Molecular Devices SpectraMax M2E provides powerful multi detection reading for microplates and cuvettes in a flexible, dual monochromator, easy to use instrument. The M2e plate reader is a great choice for applications in DNA, RNA, and Protein quantitation and purity checks, enzyme kinetics, drug dissolutions profiles, cytotoxicity assays, and many more. This multi-mode plate reader features dual cuvette ports and is compatible with microplates from 6 to 384 wells. The built-in cuvette ports make for easy conversion and optimization of low-throughput assays into medium-high-throughput microplate assays. The Spectramx M2e has a broad wavelength range of 250-850 nm, and detects in absorbance, fluorescence intensity, top and bottom fluorescence readings as primary modes, with time-resolved fluorescence, and luminescence as secondary modes.
The SpectraMax M2E Microplate Reader is low-cost to operate, thanks to the dual monochromator platform, which eliminates the need for expensive add on excitation or emission filters. The already incorporated filter wheel and scanning monochromators define the optimal excitations and emission settings, with the PathCheck sensor to measure the optical pathlength of samples in a microplate. The PathCheck sensor allows for homogeneous and heterogeneous microplate assays by getting rid of standard curves and detecting compounds with known extinction coefficient, allowing absorbance to calculate the concentration within the sample. This flexible plate reader system automatically normalizes the well absorbance to a cuvette equivalent of 1cm. The Spectramax M2e can also act as an ELISA Reader and comes with a built-in assay which is easily accessible through the SoftMax Pro software. The SpectraMax M2E Microplate Reader can do Endpoint, Kinetic, Spectrum, and area-well scanning read types. This microplate reader can also operate as well as a dedicated spectrofluorometer, or spectrophotometer. In addition, the system can also act as an ELISA Reader and comes with built-in assay which is easily accessible through the SoftMax Pro software.
SpectraMax M2E Plate Reader Specifications:
Absorbance photometric performance | |
Wavelength selection | Monochromator, tunable in 1 nm increments |
Wavelength range | 200–1000 nm |
Wavelength bandwidth | ≤ 4.0 nm |
Wavelength accuracy | ±2.0 nm |
Wavelength repeatability | ±0.2 nm |
Photometric range | 0–4.0 OD |
Photometric resolution | 0.001 OD |
Photometric accuracy (microplate) | < ±0.006 OD ±1.0%, 0–2 OD |
Photometric accuracy (cuvette) | < ±0.005 OD ±1.0%, 0–2 OD |
Photometric precision | < ±0.003 OD ±1.0%, 0–2 OD |
Baseline flatness | < 0.001 OD |
Stray light | < 0.05% @ 230 nm |
Fluorescence photometric performance | |
Dual monochromators | 1 nm increment selection |
EX Wavelength range | 250–850 nm |
EM Wavelength range | 250–850 nm |
Wavelength bandwidth (EX, EM) | 9 nm |
Top-read optimized sensitivity | 5 pM (fluorescein) in 96 wells |
Top-read guaranteed sensitivity | 15 pM (fluorescein) in 96 wells |
Bottom-read optimized sensitivity | 10 pM (fluorescein) in 96 wells |
Bottom-read guaranteed sensitivity | 25 pM (fluorescein) in 96 wells |
Time-Resolved Fluorescence (secondary mode) | |
Wavelength range | 250–850 nm |
Data collection | 50–1450 µsec., 200 µsec. increments |
Guaranteed sensitivity | 7 pM Eu-chelate |
Luminescence (secondary mode) | |
Wavelength range (M2E) | 250–850 nm |
Guaranteed sensitivity | 50 fM alkaline phosphatase |
General photometric performance | |
Plate formats | 6, 12, 24, 48, 96, 384 wells |
Light source | Xenon flash lamp (1 joule/flash) |
Detector | Photomultiplier tube (PMT) |
Read time** 96 wells: 384 wells: | Abs 18 sec., FI 15 sec. Abs 49 sec., FI 45 sec. |
Shaker time | 0 to 999 seconds |
Temperature control | Ambient +4°C to 45°C |
Temperature uniformity | < 1°C at 37°C set point |
Temperature accuracy | ±1°C at 37°C set point |
General specifications | |
Power Consumption | < 125 watts |
Power | 100–240 Vac, 3 A, 50/60 Hz |
Dimensions | 23” x 16” x 9” |
Weight | 35lbs |
- SpectraMax M2e Plate Reader | MSRP: $77,193.16
- Laptop Running SoftMax Pro 6
- Absorbance Validation Report
- Fluorescence Validation Report
- Communication Cable
- Bottom Fluorescence Purple Plate Adapter
- SpectraMax M2 User Guide
- 120V Power Cord
- 30-Day Warranty
This Molecular Devices SpectraMax M2E Microplate Reader has been fully inspected and comes with current validations reports. All of our lab equipment comes backed with our standard warranty. Should you have any questions about this or any of our other microplate readers, please don't hesitate to contact us.