Agilent HPLC 1100 Series Chromatography System video
Agilent 1100 HPLC System with DAD
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Agilent 1100 HPLC with VWD or DAD

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Description

Agilent 1100 HPLC

The Agilent 1100 HPLC offers you a customizable and highly accurate chromatography system for HPLC analysis. The Agilent series is robust and versatile with each component operating seamlessly in concert with the others or as standalone instruments controlled by a PC. These HPLC stacks come configured with components such a Variable Wavelength detector, Colcom, Autosampler, a pump, and a micro vacuum degasser. The Agilent G1312A Binary Pump provides high-pressure mixing and great gradient generation with a two-pump assembly for the Agilent 1100 HPLC, that work together, metering solvent and delivering to the high-pressure side, generating up to 400 Barr in pressure. An Agilent G1311A Quaternary Pump features a four pump gradient system, with enough space for 4 one-liter bottles.

The Agilent 1100 Series G1314A VWD is a high-performance Variable Wavelength Detector, that can be used independently or added as a component to an 1100 HPLC system. This VWD employs a deuterium lamp that provides high intensity and a low detection limit, with a wavelength range of 190-600 nm. The G1316A Colcom is a temperature-controlled column compartment built to handle Peltier heating and cooling and holds up to three 30cm columns. The Agilent Vacuum Degassers create a stable low flow rate through a 4-channel container, 4 tube membranes, and a vacuum pump.

An Agilent G1315A DAD is a diode array detector designed for maximum optical performance, this detector has a flexible slit setting from fine bands to highest sensitivity, with settings from 1 to 16 nm allowing users to customize and optimize their desired sensitivity, linearity, and spectral resolution. The fine structure of benzene can be revealed easily with this DAD at 1nm, even at 0.7mAUFS. The broad slit of 8 or 16nm can be chosen to minimize baseline noise thus enhancing sensitivity. The Agilent 1200 Series G1315B DAD features long-life deuterium and tungsten lamps for a wavelength range of 190-950mm and can handle 5 wavelengths simultaneously without loss of sensitivity.

Data acquired by this Chromatography system can is then processed with the controlling Chemstation software, with configurable reports and in-depth analysis including integration, quantification, and reporting, and it also allows you to automate and customize your whole instrument use.

Specifications

Agilent 1100 HPLC with VWD Specifications:

G1314A VWD Detector
Detection type Double-beam photometer
Light source Deuterium lamp
Wavelength range 190-600 nm
Short term noise (ASTM) ± 0.75 × 10-5 AU at 254 nm
Drift 3 × 10-4 AU/hr at 254 nm
Linearity > 2 AU (5%) upper limit
Wavelength accuracy ± 1 nm Self-calibration with deuterium lines, verification with holmium oxide filter
Bandwidth 6.5 nm typical
Flow cells Standard: 14-µl volume, 10-mm cell path length, and 40 bar (588 psi) pressure maximum High pressure: 14-µl volume, 10-mm cell path length and 400 bar (5880 psi) pressure maximum Micro: 1-µl volume, 5-mm cell path length and 40 bar (588 psi) pressure maximum Semimicro: 5-µl volume, 6-mm cell path length, and 40 bar (588 psi) pressure maximum
Analog outputs Recorder/integrator: 100 mV or 1 V, output range 0.001 – 2 AU, one output
Agilent G1316A Colcom Thermostated Column Compartment Specifications:
Temperature Range 10° below ambient to 80° C
Temperature Stability ± 0.15°C
Temperature Accuracy ± 0.8°C
Column Capacity Three 30cm columns
Warm Up/Cool Down 5 mins from ambient to 40°C
Dead Volume 3 µl left heat exchanger

6 µl right heat exchanger
G1329A Auto Sampler
Metering device Metering pump in high-pressure flow
Injection range  0.1 – 100 µl in 0.1 µl increments
Replicate injections 1 – 99 from one vial
Precision < 0.25% RSD from 5 – 100 µl, < 1% RSD 1 – 5 µl variable volume
Minimum sample volume 1 µl from 5 µl sample in 100 µl microvial, or 1 µl from 10 µl sample in 300 µl microvial
Carryover Typically < 0.1%, < 0.05% with external needle cleaning
Sample viscosity range 0.2 – 50 cp
Sample capacity 100 × 2-ml vials in 1 tray 40 × 2-ml vials in ½ tray 15 × 6-ml vials in ½ tray Agilent vials only)
Injection cycle time Typically 50 s depending on draw speed and injection volume
G1312A Binary Pump
Setable flow range Setpoints 0.001 – 5 ml/min, in 0.001 ml/min increments
Flow range 0.1 – 5.0 ml/min
Flow accuracy ± 1% or 10 µl/min whatever is greater
Pressure Operating range  0 400 bar (0 – 5880 psi) up to 5 ml/min
Pressure pulsation < 2 % amplitude (typically < 1 %), at 1 ml/min isopropanol, at all pressures > 1 MPa
Compressibility compensation User-selectable, based on mobile phase compressibility
Recommended pH range 1.0 – 12.5, solvents with pH < 2.3 should not contain acids which attack stainless steel
Gradient formation High-pressure binary mixing, delay volume 180 – 480 µl without a mixer, 600 – 900 µl with mixer, dependent on back pressure
Vacuum Degasser G1379A
Composition range  1 – 99 % or 5 µl/min per channel, whatever is greater
Setable flow range Setpoints 0.001 – 5 ml/min, in 0.001 ml/min increments
Flow range 0.1 – 5.0 ml/min
Flow accuracy ± 1% or 10 µl/min whatever is greater
Pressure Operating range  0 400 bar (0 – 5880 psi) up to 5 ml/min
Pressure pulsation < 2 % amplitude (typically < 1 %), at 1 ml/min isopropanol, at all pressures > 1 MPa
Compressibility compensation User-selectable, based on mobile phase compressibility
Recommended pH range 1.0 – 12.5, solvents with pH < 2.3 should not contain acids which attack stainless steel
Gradient formation High-pressure binary mixing, delay volume 180 – 480 µl without a mixer, 600 – 900 µl with mixer, dependent on back pressure
Composition range  1 – 99 % or 5 µl/min per channel, whatever is greater
Composition precision 0.5% absolute
Agilent G1330B ALS Thermostat
Temperature range 4 °C to 40 °C in 1 ° increments
Temperature accuracy at ambient temperatures < 25 °C and humidity < 50 % 3 °C to 8 °C at a setpoint of 4 °C*
Temperature accuracy at ambient temperatures <30 °C and humidity <60 % 3 °C to 9 °C at a setpoint of 4 °C*
G1315A DAD Detector
Wavelength Range: 190 – 950 nm
Light Source: Deuterium and Tungsten Lamps
Detection Type: 1024 – element photodiode array
ASTM single & multi-wavelength: ± 1° x 10-5 AU at 254 and 750 nm
Drift: 2° x 10-3 AU / hr at 254 nm
Linear Absorbance Range: > 2 AU (upper limit)
Wavelength Accuracy: ± 1 nm
Wavelength Bunching: 1 – 400 nm
Diode Width: < 1 nm
Flow Cells: Standard: 13µl volumne 10 nm cell path length and 120 bar (1760 psi) pressure maximum

Micro: 2µl volume, 3mm cell path length and 120 bar (1760 psi) pressue maximum

Semi Micro: 5µl volume. 6 mm cell path length and 120 (1760 psi) pressure maximum
Analog Outputs: Recorder / Integrator: 10mV or 1V, output range 0.001 – 2AU, two outputs
Control and Data Evaluation: Agilent Chemstation for LC
Slit Width: 1, 2, 4, 8, 16mm
Communication Types: CAN, GBIP, RS-232C, APG, Remote: ready, start, stop and shut down
Quat Pump G1311A
Setable flow range 0.001 – 10 ml/min, in 0.001 ml/min increments
Flow range 0.2 – 10.0 ml/min
Flow accuracy ± 1% or 10 µl/min whatever is greater
Pressure Operating range  Operating range 0 – 40 MPa (0 – 400 bar, 0 – 5880 psi) up to 5 ml/min

Operating range 0 – 20 MPa (0 – 200 bar, 0 – 2950 psi) up to 10 ml/min
Pressure pulsation < 2 % amplitude (typically < 1 %), at 1 ml/min isopropanol, at all pressures > 1 MPa (10 bar
Compressibility compensation User-selectable, based on mobile phase compressibility
Recommended pH range 1.0 – 12.5, solvents with pH < 2.3 should not contain acids which attack stainless steel
Gradient formation Low pressure quaternary mixing/gradient capability using proprietary high-speed proportioning valve Delay volume 800 – 1100 µl, dependent on back pressure
Composition range 0 – 95 % or 5 – 100 %, user selectable
Composition precision < 0.2 % RSD, at 0.2 and 1 ml/min

Included

Included with HPLC

Agilent HPLC 1100 Series Chromatography System
Laptop Running Chemstation & Lab Adviser
System Validation Reports
G1315A DAD Detector
G1311A Quat Pump
G1329A ALS Autosampler
G1316A Colcom
G1379A Vacuum Degasser
Solvent Tray Holder
CAN Communication Cables
120V Power Cords
User Manual
30-Day Warranty

Notes

Technical Notes

This Agilent 1100 HPLC has been fully inspected and comes with validation reports for every component. All of our lab equipment comes backed with our standard warranty. Should you have any questions about this or any of our Chromatography Equipment, please don’t hesitate to contact us.

Video

Agilent 1100 HPLC with DAD Video

Agilent 1100 HPLC ID 14311 for sale