Molecular Devices SpectraMax M2E Microplate Reader

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Description

Molecular Devices SpectraMax M2E

The Molecular Devices SpectraMax M2E provides powerful multi detection reading for microplates and cuvettes in a flexible, dual monochromator, easy-to-use instrument. The M2e plate reader is a great choice for applications in DNA, RNA, and Protein quantitation and purity checks, enzyme kinetics, drug dissolutions profiles, cytotoxicity assays, and many more. This multi-mode plate reader features dual cuvette ports and is compatible with microplates from 6 to 384 wells. The built-in cuvette ports make for easy conversion and optimization of low-throughput assays into medium-high-throughput microplate assays. The SpectraMax M2e has a broad wavelength range of 250-850 nm, and detects absorbance, fluorescence intensity, top and bottom fluorescence readings as primary modes, with time-resolved fluorescence, and luminescence as secondary modes.

The SpectraMax M2E plate Reader is low-cost to operate, thanks to the dual monochromator platform, which eliminates the need for expensive add-on excitation or emission filters. The already incorporated filter wheel and scanning monochromators define the optimal excitations and emission settings, with the PathCheck sensor to measure the optical path length of samples in a microplate. The PathCheck sensor allows for homogeneous and heterogeneous microplate assays by getting rid of standard curves and detecting compounds with known extinction coefficients, allowing absorbance to calculate the concentration within the sample.

This flexible plate reader system automatically normalizes the good absorbance to a cuvette equivalent of 1cm. The SpectraMax M2e can also act as an ELISA Reader and comes with a built-in assay which is easily accessible through the SoftMax Pro software. The SpectraMax M2E Microplate Reader can do Endpoint, Kinetic, Spectrum, and area-well scanning read types. This microplate reader can also operate as well as a dedicated spectrofluorometer, or spectrophotometer. In addition, the system can also act as an ELISA Reader and comes with a built-in assay which is easily accessible through the SoftMax Pro software.

Specifications

SpectraMax M2E Plate Reader Specifications:

Absorbance photometric performance
Wavelength selection Monochromator, tunable in 1 nm increments
Wavelength range 200-1000 nm
Wavelength bandwidth 4.0 nm
Wavelength accuracy ±2.0 nm
Wavelength repeatability ±0.2 nm
Photometric range 0-4.0 OD
Photometric resolution 0.001 OD
Photometric accuracy (microplate) < ±0.006 OD ±1.0%, 0-2 OD
Photometric accuracy (cuvette) < ±0.005 OD ±1.0%, 0-2 OD
Photometric precision < ±0.003 OD ±1.0%, 0-2 OD
Baseline flatness < 0.001 OD
Stray light < 0.05% @ 230 nm
Fluorescence photometric performance
Dual monochromators 1 nm increment selection
EX Wavelength range 250-850 nm
EM Wavelength range 250-850 nm
Wavelength bandwidth (EX, EM) 9 nm
Top-read optimized sensitivity 5 pM (fluorescein) in 96 wells
Top-read guaranteed sensitivity 15 pM (fluorescein) in 96 wells
Bottom-read optimized sensitivity 10 pM (fluorescein) in 96 wells
Bottom-read guaranteed sensitivity 25 pM (fluorescein) in 96 wells
Time-Resolved Fluorescence (secondary mode)
Wavelength range 250-850 nm
Data collection 50-1450 µsec., 200 µsec. increments
Guaranteed sensitivity 7 pM Eu-chelate
Luminescence (secondary mode)
Wavelength range (M2E) 250-850 nm
Guaranteed sensitivity 50 fM alkaline phosphatase
General photometric performance
Plate formats 6, 12, 24, 48, 96, 384 wells
Light source Xenon flash lamp (1 joule/flash)
Detector Photomultiplier tube (PMT)
Read time** 96 wells: 384 wells: Abs 18 sec., FI 15 sec. Abs 49 sec., FI 45 sec.
Shaker time 0 to 999 seconds
Temperature control Ambient +4°C to 45°C
Temperature uniformity < 1°C at 37°C set point
Temperature accuracy ±1°C at 37°C set point
General specifications
Power Consumption  < 125 watts
Power 100-240 Vac, 3 A, 50/60 Hz
Dimensions 23 x 16 x 9 inches
Weight 35lbs

Included

Included with SpectraMax M2E Plate Reader

Molecular Devices SpectraMax M2e Multi-Detection Microplate Reader | MSRP:
Laptop Running SoftMax Pro 6.2.2 Software
Absorbance Validation Report
Fluorescence Validation Report
User Manual
Communication Cable
120V Power Cord (4A)
30-Day Warranty

Notes

Technical Notes

This Molecular Devices SpectraMax M2E Microplate Reader has been fully inspected and comes with current validations reports. All of our lab equipment comes backed with our standard warranty. Should you have any questions, please don’t hesitate to contact us.

Video

SpectraMax M2E Plate Reader Video

Molecular Devices SpectraMax M2e ID 17998