- Description
- Specifications
- Included
- Notes
- Video
Description
Description
Molecular Devices Spectramax M5 Microplate Reader
The Spectramax M5 Plate Reader brings you the power and precision of a dedicated single-mode plate reader but with the space-saving flexibility of 5 detection modes and cuvette capabilities in one unit. This microplate reader handles Absorbance, Fluorescence Intensity, luminescence, time-resolved fluorescence, and fluorescence polarization and comes with 140 preprogrammed protocols to get you up and running fast. This microplate reader uses two monochromators, allowing users to choose their optimal emission or excitation and eliminating the need to change filters. The Spectramax 5 covers formats from cuvettes to 6-, 12-, 24-, 48-, 96-, and 384-well microplates and incorporates a triple-mode cuvette port, accurate temperature control, and microplate shaking.
The Molecular Devices Spectramax M5 Plate Reader uses a dedicated photomultiplier tube to reduce stray light and boost the best signal to noise. For luminescence reading, Instead of lenses, this Microplate Reader uses elliptical mirrors that maximize transmission while minimizing wavelength distortion. The Spectramax 5 utilizes powerful PathCheck Sensory technology to measure the depth of samples without being temperature-dependent. In addition, when used with SoftMax Pro, the Path Check Sensor of this microplate reader will normalize the well absorbance, letting users calculate directly without absorptive compounds. This microplate is also robot-compatible to help boost productivity and automation, and it features customizable spreadsheets and powerful graphing tools.
Specifications
Specifications
Molecular Devices Spectramax M5 Specifications:
| Read modes | Absorbance
Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization |
| Robotic-compatible | Yes |
| Plate formats | 6, 12, 24, 48, 96, 384 wells |
| Light source | Xenon Flash Lamp (1 joule/flash) |
| Detectors | 2 photomultiplier tubes (PMT) |
| Shaker time | 0 to 999 seconds |
| Temp. control | 2°C above ambient to 60°C |
| Temp. uniformity | < 1°C at 37°C set point |
| Temp. accuracy | ±1°C at 37°C set point |
| Readings | Endpoint, Kinetic Spectral Scanning |
| Standard Read Times (minutes: seconds)* 96 wells 384 wells
|
Absorbance 0:18 0:49
Fluorescence Intensity 0:17 0:48 Luminescence 2:00 7:00 |
| Absorbance Photometric Performance | |
| Reading Capabilities | Cuvette or microplate |
| Wavelength range | 200–1000 nm |
| Wavelength selection | Monochromator, tunable in 1.0 nm increments |
| Wavelength bandwidth | ≤ 4.0 nm |
| Wavelength accuracy | ±2.0 nm |
| Wavelength repeatability | ±0.2 nm |
| Photometric range | 0–4.0 OD |
| Photometric resolution | 0.001 OD |
| Photometric accuracy | (microplate) < ±0.006 OD ±1.0%, 0–2 OD
(cuvette) < ±0.005 OD ±1.0%, 0–2 OD
|
| Photometric precision | < ±0.003 OD ±1.0%, 0–2 OD |
| Stray light | < 0.05% @ 230 nm |
| Fluorescence Intensity Performance | Top or bottom |
| Wavelength range | 250–850 nm |
| Wavelength selection | Monochromators, tunable in 1.0 nm increments |
| Bandwidth (EX, EM) | 9 nm, 15 nm |
| Optimized sensitivity | ≤ 1 pM fluorescein in 96 wells,
≤ 1.5pM in 384 wells |
| Guaranteed sensitivity | < 5 pM fluorescein in 96 wells or cuvette,
< 20 pM in 384 wells |
| Luminescence Performance | Top or Bottom |
| Wavelength selection | Choice of simultaneous detection of all wavelengths or selection via monochromator, tunable in 1.0 nm increments |
| Wavelength range | 250–850 nm |
| Optimized sensitivity | ≤ 43pM ATP in 96 wells |
| Guaranteed sensitivity | ≤ 75pM ATP in 96 wells |
| Dynamic range | > 6 decades |
| Cross-talk | < 0.3% in white 96- and 384-well Microplates |
| Time Resolved Fluorecesnce Performance | Top or Bottom of a Microplate |
| Wavelength selection Monochromators, | tunable in 1.0 nm increments |
| Bandwidth (EX, EM) | 9 nm, 15 nm |
| Precision data collection | 1–100 flashes, delay of 0–600 µsec. before read, integration time selectable between 50–1500 µsec. |
| Optimized sensitivity | ≤ 10 fM europium in 96 |
| Guaranteed sensitivity | ≤ 100 fM europium in 96 or 384 wells |
| Fluorescence Polarization Performance | |
| Wavelength range | 300–750 nm |
| Wavelength selection | Monochromators, tunable in 1.0 nm increments |
| Bandwidth (EX, EM) | 9 nm, 15 nm |
| Optimized Precision | ≤ 3.5 mP standard deviation at 1 nM fluorescein in 96 wells |
| Guaranteed Precision | < 5 mP standard deviation at 1 nM fluorescein in 96 wells |
| Dimensions | 22x16x9” |
| Weight | 36 lbs |
| Power | < 420 watts, 100–240 VAC, 3.5 A, 50/60Hz |
Included
Included
Included with the Spectramax M5
Please see individual SKU’s for a complete list of included items.
Notes
Notes
Technical Notes
This system has been fully calibrated and passed validated with a clean bill of health. Validations can be seen in the photo gallery above. All of our lab equipment comes backed by our standard warranty. Should you have any questions, please don’t hesitate to contact us.
Video
Video
Spectramax M5 Microplate Reader Video
ID 19463-20112-24230
Molecular Devices SpectraMax M5 ID 19115
ID 23739
Molecular Device SpectraMax M5 Microplate Reader ID 23739
