Build Your Own Centrifuge For Under Fifty Dollars | DIY Centrifuge Projectadmin
In one of the past blogs “How to Build Your Own PCR for Under $50 dollars” had such a great response from our readership; we thought we’d share this DIY centrifuge project. Below is an easy-to-follow five step instructions on how to build your own lab grade centrifuge in less than one hour for around fifty dollars. We hope you enjoy! For any gear head ready to tackle molecular biology, the centrifuge is an essential laboratory tool. Its super-rapid spin supplies the right G-forces to neatly separate biological materials—including cells, proteins, and DNA—from a liquid. University-grade centrifuges sell for about $2,000, but synthetic biology enthusiast and inventor Cathal Garvey figured out how to build one with a Dremel tool and a 3-D–printed wheel. The combination can spin samples up to 33,000 rpm, creating forces 50,000 times stronger than Earth’s gravitational pull. The 3-D–printed wheel looks deceptively simple, with six slots for standard 1.5-microliter Eppendorf tubes, oriented horizontally. Garvey says his early prototypes often deformed which cracked and shot off tubes like oversize plastic bullets. “People said I was mad,” Garvey says. Yet he prevailed, reshaping the wheel’s slots to safely cradle the tubes’ thick rims. “I’ve never had a tube eject since,” he says. Still, Garvey advises using extreme caution—and proper eye protection—when running what he’s dubbed the Dremel-fuge.
INSTRUCTIONS 1.) Download schematics for the Dremel-fuge wheel here. Two designs are available; pick the one that fits a Dremel 300 (not a standard drill). 2) 3-D–print the wheel in ABS plastic, using hexagonal infill to strengthen it. If you lack a 3-D printer, order the wheel from Shapeways.com for about $50. 3) Screw a rotary-tool disc holder into the wheel’s axis, and attach it to a Dremel. 4) Fit any tubes containing a biological sample into the wheel, ensuring each has a counterweight: a tube on the opposite side with an equal amount of liquid. Otherwise, they might damage the wheel and the Dremel and make a mess. 5) Hold the Dremel-fuge in a Styrofoam cooler, and let ’er rip! The foam will absorb the impact if a tube pops off.
WARNING: Use shatterproof eye protection, and operate the Dremelfuge inside a sturdy container, as the device can throw off hunks of plastic at dangerously high speeds.
TEST RUN The cells lining your inner cheeks constantly slough off into saliva but are too small to see individually,making them perfect candidates for testing the Dremel-fuge. 1) Swab a Q-tip inside your mouth for about 10 seconds to grab some cheek cells. 2) Dip the cotton tip repeatedly in a tube filled with isotonic saline solution to dislodge the cells. 3) Fill a second tube to the same level as the other one. Insert the tubes in opposite slots in the 3-D–printed wheel. 4) Run for one minute at the Dremel’s second setting (about 10,000 rpm). You’ll see a whitish pellet at the bottom of the tubes. Voilà! Isolated cheek cells.